2021

Yamanaka S, Murai H, Saito D, Abe G, Tokunaga E, Iwasaki T, Takahashi H, Takeda H, Suzuki T, Shibata N, Tamura K, Sawasaki T. Thalidomide and its metabolite 5-hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF. EMBO J. 2021 Feb 15;40(4):e105375. doi: 10.15252/embj.2020105375. LinkIconOpenAccess

Nakabayashi O, Takahashi H, Moriwaki K, Komazawa-Sakon S, Ohtake F, Murai S, Tsuchiya Y, Koyahara Y, Saeki Y, Yoshida Y, Yamazaki S, Tokunaga F, Sawasaki T, Nakano H. MIND bomb 2 prevents RIPK1 kinase activity-dependent and -independent apoptosis through ubiquitylation of cFLIPL. Commun Biol. 2021 Jan 19;4(1):80. doi: 10.1038/s42003-020-01603-y. LinkIconOpenAccess

2020

Kurokawa K, Kobayashi J, Nemoto K, Nozawa A, Sawasaki T, Nakatsuka T, Yamagishi M. Expression of LhFT1, the Flowering Inducer of Asiatic Hybrid Lily, in the Bulb Scales. Front Plant Sci. 2020 Nov 9;11:570915. doi: 10.3389/fpls.2020.570915. LinkIconOpenAccess

Yamanaka S, Shoya Y. Matsuoka S, Nishida-Fukuda H, Shibata N, Sawasaki T. An IMiD-induced SALL4 degron system for selective degradation of target proteins. Commun Biol. 2020 Sep 18;3:515. doi: 10.1038/s42003-020-01240-5. LinkIconOpenAccess

Furihata H#, Yamanaka S#, Honda T, Miyauchi Y, Asano A, Shibata N, Tanokura M*, Sawasaki T*, Miyakawa T*. Structural bases of IMiD selectivity that emerges by 5-hydroxythalidomide. Nat Commun. 2020 Sep 14;11(1):4578. doi: 10.1038/s41467-020-18488-4. (#co-first author, *corresponding author)LinkIconOpenAccess

Inoue SI, Kaiserli E, Zhao X, Waksman T, Takemiya A, Okumura M, Takahashi H, Seki M, Shinozaki K, Endo Y, Sawasaki T, Kinoshita T, Zhang X, Christie JM, Shimazaki KI. CIPK23 regulates blue light-dependent stomatal opening in Arabidopsis thaliana. Plant J. 2020 Nov;104(3):679-692. doi: 10.1111/tpj.14955.
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Kaneko N, Kurata M, Yamamoto T, Shigemura T, Agematsu K, Yamazaki T, Takeda H, Sawasaki T, Koga T, Kawakami A, Yachie A, Migita K, Yoshiura KI, Urano T, Masumoto J. KN3014, a piperidine-containing small compound, inhibits auto-secretion of IL-1β from PBMCs in a patient with Muckle-Wells syndrome. Sci Rep. 2020 Aug 11;10(1):13562. doi: 10.1038/s41598-020-70513-0.LinkIconOpenAccess

Takahashi H*, Yamanaka S, Kuwada S, Higaki K, Kido K, Sato Y, Fukai S, Tokunaga F, and Sawasaki T*. A human DUB protein array for clarification of linkage specificity of polyubiquitin chain and application to evaluation of its inhibitors. Biomedicines. 2020 Jun 4;8(6):E152. doi: 10.3390/biomedicines8060152.LinkIconOpenAccess

Kido K, Yamanaka S, Nakano S, Motani K, Shinohara S, Nozawa A, Kosako H, Ito S, Sawasaki T. AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions. eLife. 2020 May 11;9. pii: e54983. doi: 10.7554/eLife.54983. LinkIconOpenAccess

Uemura T, Hachisu M, Desaki Y, Ito A, Hoshino R, Sano Y, Nozawa A, Mujiono K, Galis I, Yoshida A, Nemoto K, Miura S, Nishiyama M, Nishiyama C, Horito S, Sawasaki T, Arimura GI. Soy and Arabidopsis receptor-like kinases respond to polysaccharide signals from Spodoptera species and mediate herbivore resistance. Commun Biol. 2020 May 8;3(1):224. doi: 10.1038/s42003-020-0959-4.LinkIconOpenAccess

Suzuki Y, Liu S, Ogasawara T, Sawasaki T, Takasaki Y, Yorozuya T, Mogi M. A novel MRGPRX2-targeting antagonistic DNA aptamer inhibits histamine release and prevents mast cell-mediated anaphylaxis. Eur J Pharmacol. 2020 Apr 19;878:173104. doi: 10.1016/j.ejphar.2020.173104.LinkIconweb

Miyoshi S, Tokunaga S, Ozawa T, Takeda H, Aono M, Miyoshi T, Kishi H, Muraguchi A, Shimizu SI, Nozawa A*, Sawasaki T*. Production of a rabbit monoclonal antibody for highly sensitive detection of citrus mosaic virus and related viruses. PLoS One. 2020 Apr 15;15(4):e0229196. doi: 10.1371/journal.pone.0229196.LinkIconOpenAccess

Yamanaka S, Sato Y, Oikawa D, Goto E, Fukai S, Tokunaga F, Takahashi H, Sawasaki T. Subquinocin, a small molecule inhibitor of CYLD and USP-family deubiquitinating enzymes, promotes NF-κB signaling. Biochem Biophys Res Commun. 2020 Mar 26;524(1):1-7. doi: 10.1016/j.bbrc.2019.12.049. LinkIconOpenAccess

Hayashi M, Sugimoto H, Takahashi H, Seki M, Shinozaki K, Sawasaki T, Kinoshita T, Inoue SI. Raf-like kinases CBC1 and CBC2 negatively regulate stomatal opening by negatively regulating plasma membrane H+-ATPase phosphorylation in Arabidopsis. Photochem Photobiol Sci. 2020 Jan 22;19(1):88-98. doi: 10.1039/c9pp00329k. LinkIconweb

高橋宏隆, 山中聡士, 徳永文稔, 澤崎達也. ヒト脱ユビキチン化酵素タンパク質アレイの開発とその応用例, Journal of Japanese Biochemical Society (生化学) 2020 92(1): 64-74. doi:10.14952/SEIKAGAKU.2020.920064LinkIconweb

高橋 宏隆, 山中 聡士. コムギ無細胞合成系による脱ユビキチン化酵素アレイの作製とDUB阻害剤開発への応用 2020年 56巻, 1号, p.51-55. doi: 10.14894/faruawpsj.56.1_51LinkIconweb

2019

Morishita R, Sugiyama S, Denda M, Tokunaga S, Kido K, Shioya R, Ozawa S, Sawasaki T. CF-PA2Vtech: a cell-free human protein array technology for antibody validation against human proteins. Sci Rep. 2019 Dec 18;9(1):19349. doi: 10.1038/s41598-019-55785-5.LinkIconOpenAccess

Nomura S, Takahashi H, Suzuki J, Kuwahara M, Yamashita M, Sawasaki T. Pyrrothiogatain acts as an inhibitor of GATA family proteins and inhibits Th2 cell differentiation in vitro. Sci Rep. 2019 Nov 22;9(1):17335. doi: 10.1038/s41598-019-53856-1.LinkIconOpenAccess

Uematsu A, Kido K, Takahashi H, Takahashi C, Yanagihara Y, Saeki N, Yoshida S, Maekawa M, Honda M, Kai T, Shimizu K, Higashiyama S, Imai Y, Tokunaga F, Sawasaki T. The E3 ubiquitin ligase MIB2 enhances inflammation by degrading the deubiquitinating enzyme CYLD. J Biol Chem. 2019 Sep 20;294(38):14135-14148. doi: 10.1074/jbc.RA119.010119.LinkIconweb

Maekawa M, Hiyoshi H, Nakayama J, Kido K, Sawasaki T, Semba K, Kubota E, Joh T, Higashiyama S. Cullin-3/KCTD10 complex is essential for K27-polyubiquitination of EIF3D in human hepatocellular carcinoma HepG2 cells. Biochem Biophys Res Commun. 2019 Sep 3;516(4):1116-1122. doi: 10.1016/j.bbrc.2019.07.010.LinkIconOpenAccess

Miyamoto T, Uemura T, Nemoto K, Daito M, Nozawa A, Sawasaki T, Arimura GI. Tyrosine Kinase-Dependent Defense Responses Against Herbivory in Arabidopsis. Front Plant Sci. 2019 Jun 12;10:776. doi: 10.3389/fpls.2019.00776.LinkIconOpenAccess

Miyakawa K, Matsunaga S, Yokoyama M, Nomaguchi M, Kimura Y, Nishi M, Kimura H, Sato H, Hirano H, Tamura T, Akari H, Miura T, Adachi A, Sawasaki T, Yamamoto N, Ryo A. PIM kinases facilitate lentiviral evasion from SAMHD1 restriction via Vpx phosphorylation. Nat Commun. 2019 Apr 23;10(1):1844. doi: 10.1038/s41467-019-09867-7.LinkIconOpenAccess

Ali MRM, Uemura T, Ramadan A, Adachi K, Nemoto K, Nozawa A, Hoshino R, Abe H, Sawasaki T, Arimura GI. The Ring-type E3 Ubiquitin Ligase JUL1 Targets the VQ-motif Protein JAV1 to Coordinate Jasmonate Signaling. Plant Physiol. 2019 Apr;179(4):1273-1284. doi: 10.1104/pp.18.00715.LinkIconOpenAccess

2018

Nakanishi A, Kaneko N, Takeda H, Sawasaki T, Morikawa S, Zhou W, Kurata M, Yamamoto T, Akbar SMF, Zako T, Masumoto J. Amyloid β directly interacts with NLRP3 to initiate inflammasome activation: identification of an intrinsic NLRP3 ligand in a cell-free system. Inflamm Regen. 2018 Nov 12;38:27. doi: 10.1186/s41232-018-0085-6.LinkIconweb

Yonezawa T, Takahashi H, Shikata S, Sawasaki T, Kitamura T, Goyama S. The ubiquitin ligase RNF38 promotes RUNX1 ubiquitination and enhances RUNX1-mediated suppression of erythroid transcription program. Biochem Biophys Res Commun. 2018 Nov 2;505(3):905-909. doi: 10.1016/j.bbrc.2018.10.006.LinkIconweb

Hashimoto Y, Zhou W, Hamauchi K, Shirakura K, Doi T, Yagi K, Sawasaki T, Okada Y, Kondoh M, Takeda H.Engineered membrane protein antigens successfully induce antibodies against extracellular regions of claudin-5. Sci Rep. 2018 May 30;8(1):8383. doi: 10.1038/s41598-018-26560-9.LinkIconOpenAccess

Miura T, Takeo S, Ntege EH, Otsuki H, Sawasaki T, Ishino T, Takashima E, Tsuboi T. The malaria parasite RhopH protein complex interacts with erythrocyte calmyrin identified from a comprehensive erythrocyte protein library. Biochem Biophys Res Commun. 2018 Apr 10. pii: S0006-291X(18)30819-2. doi: 10.1016/j.bbrc.2018.04.056. In press LinkIconweb

Nemoto K, Kagawa M, Nozawa A, Hasegawa Y, Hayashi M, Imai K, Tomii K, Sawasaki T. Identification of new abscisic acid receptor agonists using a wheat cell-free based drug screening system. Sci Rep. 2018 Mar 9;8(1):4268. doi: 10.1038/s41598-018-22538-9. LinkIconOpenAccess

Suzuki Y, Ogasawara T, Tanaka Y, Takeda H, Sawasaki T, Mogi M, Liu S, Maeyama K. Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The Pharmacological Analysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes. Front Pharmacol. 2018 Feb 6;9:38. doi: 10.3389/fphar.2018.00038. eCollection 2018.LinkIconOpenAccess

Uematsu A, Kido K, Manabe E, Takeda H, Takahashi H, Hayashi M, Imai Y, Sawasaki T. DANFIN functions as an inhibitor of transcription factor NF-κB and potentiates the antitumor effect of bortezomib in multiple myeloma. Biochem Biophys Res Commun.
2018 Jan 15;495(3):2289-2295. doi: 10.1016/j.bbrc.2017.12.142.
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根本圭一郎、澤崎達也.
チロシンリン酸化によるジベレリン応答の新しい制御機構. バイオインダストリー 2018 76(3):232-225.LinkIconweb

2017

Nemoto K, Ramadan A, Arimura GI, Imai K, Tomii K, Shinozaki K, Sawasaki T. Tyrosine phosphorylation of the GARU E3 ubiquitin ligase promotes gibberellin signalling by preventing GID1 degradation. Nat Commun. 2017 Oct 17;8(1):1004. doi: 10.1038/s41467-017-01005-5.LinkIconOpenAccess

Kaneko N, Iwasaki T, Ito Y, Takeda H, Sawasaki T, Morikawa S, Nakano N, Kurata M, Masumoto J. Applications of reconstituted inflammasomes in a cell-free system to drug discovery and elucidation of the pathogenesis of autoinflammatory diseases. Inflamm Regen. 2017 May 3;37:9. doi: 10.1186/s41232-017-0040-y.LinkIconweb

Sun S, Nakashima K, Ito M, Li Y, Chida T, Takahashi H, Watashi K, Sawasaki T, Wakita T, Suzuki T. Involvement of PUF60 in Transcriptional and Post-transcriptional Regulation of Hepatitis B Virus Pregenomic RNA Expression. Sci Rep. 2017 Oct 9;7(1):12874. doi: 10.1038/s41598-017-12497-y.LinkIconOpenAccess

Hashimoto Y, Shirakura K, Okada Y, Takeda H, Endo K, Tamura M, Watari A, Sadamura Y, Sawasaki T, Doi T, Yagi K, Kondoh M. Claudin-5-binders enhance permeation of solutes across the blood-brain barrier in a mammalian model. J Pharmacol Exp Ther. 2017 Nov;363(2):275-283. doi: 10.1124/jpet.117.243014.
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Liu S, Hasegawa H, Takemasa E, Suzuki Y, Oka K, Kiyoi T, Takeda H, Ogasawara T, Sawasaki T, Yasukawa M, Maeyama K. Efficiency and Safety of CRAC Inhibitors in Human Rheumatoid Arthritis Xenograft Models. J Immunol. 2017 Jul 17;199:1584-1595. doi: 10.4049/jimmunol.1700192. LinkIconweb

Nemoto K, Nozawa A, Yamanaka S, Nomura S, Kido K, Sawasaki T. Autophosphorylation Assays Using Plant Receptor Kinases Synthesized in Cell-Free Systems. Methods Mol Biol. 2017;1621:113-120. doi: 10.1007/978-1-4939-7063-6_11. LinkIconweb

Nozawa A, Nemoto K, Nomura S, Yamanaka S, Kido K, Sawasaki T. Cell-Free Synthesis of Plant Receptor Kinases. Methods Mol Biol. 2017;1621:37-46. doi: 10.1007/978-1-4939-7063-6_4. LinkIconweb

Takeda H, Zhou W, Kido K, Suno R, Iwasaki T, Kobayashi T, Sawasaki T. CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag. PLoS One. 2017 May 25;12(5):e0178246. doi: 10.1371/journal.pone.0178246. LinkIconOpenAccess

Yonezawa T, Takahashi H, Shikata S, Liu X, Tamura M, Asada S, Fukushima T, Fukuyama T, Tanaka Y, Sawasaki T, Kitamura T, Goyama S. The ubiquitin ligase STUB1 regulates stability and activity of RUNX1 and RUNX1-RUNX1T1.
J Biol Chem. 2017 Jul 28;292(30):12528-12541. doi: 10.1074/jbc.M117.785675. LinkIconweb

Krug SM, Hayaishi T, Iguchi D, Watari A, Takahashi A, Fromm M, Nagahama M, Takeda H, Okada Y, Sawasaki T, Doi T, Yagi K, Kondoh M. Angubindin-1, a novel paracellular absorption enhancer acting at the tricellular tight junction. J Control Release. 2017 May 18;260:1-11. doi: 10.1016/j.jconrel.2017.05.024.LinkIconweb

竹田浩之、澤崎達也.
AGIA タグシステム:細胞生物学研究に最適な高感度検出および キャプチャー用ペプチドタグ. 生化学 2017 89(2):302-307.LinkIconweb

Yamamoto T, Taira Nihira N, Yogosawa S, Aoki K, Takeda H, Sawasaki T, Yoshida K. Interaction between RNF8 and DYRK2 is required for the recruitment of DNA repair molecules to DNA double-strand breaks. FEBS Lett. 2017 Mar;591(6):842-853. doi: 10.1002/1873-3468.12596.
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Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, Tanaka K, Takahashi H, Sawasaki T, Inoue S, Oshiumi H, Seya T, Nakano H, Tanaka Y, Iwai K, Inoue JI. HTLV-1 Tax Induces Formation of the Active Macromolecular IKK Complex by Generating Lys63- and Met1-Linked Hybrid Polyubiquitin Chains. PLoS Pathog. 2017 Jan 19;13(1):e1006162. doi: 10.1371/journal.ppat.1006162.LinkIconOpenAccess

Ogawa S, Miyamoto K, Nemoto K, Sawasaki T, Yamane H, Nojiri H, Okada K. OsMYC2, an essential factor for JA-inductive sakuranetin production in rice, interacts with MYC2-like proteins that enhance its transactivation ability. Sci Rep. 2017 Jan 9;7:40175. doi: 10.1038/srep40175.LinkIconOpenAccess

2016

Takagi M, Sakamoto T, Suzuki R, Nemoto K, Obayashi T, Hirakawa T, Matsunaga TM, Kurihara D, Nariai Y, Urano T, Sawasaki T, Matsunaga S. Plant Aurora kinases interact with and phosphorylate transcription factors. J Plant Res. 2016 Nov;129(6):1165-1178.LinkIconweb

Kuwahara M, Ise W, Ochi M, Suzuki J, Kometani K, Maruyama S, Izumoto M, Matsumoto A, Takemori N, Takemori A, Shinoda K, Nakayama T, Ohara O, Yasukawa M, Sawasaki T, Kurosaki T, Yamashita M. Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat Commun. 2016 Sep 1;7:12596. doi: 10.1038/ncomms12596. LinkIconOpenAccess

Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, Tokunaga F. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016 Aug 24;7:12547. doi: 10.1038/ncomms12547. LinkIconOpenAccess

Sakamaki K, Ishii TM, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Jung SK, Matsuoka S, Koyamada K, Sawasaki T, Nagai T, Ueno N. Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage. Biochim Biophys Acta. 2016 Aug 23. pii: S0167-4889(16)30215-4. doi: 10.1016/j.bbamcr.2016.08.010. LinkIconweb

Santolini M, Sakakibara I, Gauthier M, Ribas-Aulinas F, Takahashi H, Sawasaki T, Mouly V, Concordet JP, Defossez PA, Hakim V, Maire P. MyoD reprogramming requires Six1 and Six4 homeoproteins: genome-wide cis-regulatory module analysis. Nucleic Acids Res. 2016 Jun 14. (in press) LinkIconweb

Iwasaki T, Kaneko N, Ito Y, Takeda H, Sawasaki T, Heike T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J. Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset Sarcoidosis. ScientificWorldJournal. 2016;2016:2597376. doi: 10.1155/2016/2597376.LinkIconweb

Kudoh A, Miyakawa K, Matsunaga S, Matsushima Y, Kosugi I, Kimura H, Hayakawa S, Sawasaki T, Ryo A. H11/HSPB8 Restricts HIV-2 Vpx to Restore the Anti-Viral Activity of SAMHD1. Front Microbiol. 2016 Jun 13;7:883. doi: 10.3389/fmicb.2016.00883. LinkIconweb

Yano T, Takeda H, Uematsu A, Yamanaka S, Nomura S, Nemoto K, Iwasaki T, Takahashi H, Sawasaki T. AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis. PLoS One. 2016 Jun 6;11(6):e0156716. doi: 10.1371/journal.pone.0156716. LinkIconOpenAccessLinkIcon論文概要:AGIA tag system 

Takahashi H, Uematsu A, Yamanaka S, Imamura M, Nakajima T, Doi K, Yasuoka S, Takahashi C, Takeda H, Sawasaki T. Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins. PLoS One. 2016 Jun 1;11(6):e0156718. doi: 10.1371/journal.pone.0156718. LinkIconOpenAccessLinkIcon論文概要:標的タンパク質の責任ユビキチン〜 

Suzuki J, Maruyama S, Tamauchi H, Kuwahara M, Horiuchi M, Mizuki M, Ochi M, Sawasaki T, Zhu J, Yasukawa M, Yamashita M. Gfi1, a transcriptional repressor, inhibits the induction of the T helper type 1 programme in activated CD4 T cells. Immunology. 2016 Apr;147(4):476-87. doi: 10.1111/imm.12580.LinkIconweb

Shi G, Ando T, Suzuki R, Matsuda M, Nakashima K, Ito M, Omatsu T, Oba M, Ochiai H, Kato T, Mizutani T, Sawasaki T, Wakita T, Suzuki T. Involvement of the 3' Untranslated Region in Encapsidation of the Hepatitis C Virus. PLoS Pathog. 2016 Feb 11;12(2):e1005441. doi: 10.1371/journal.ppat.1005441.
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Suzuki Y, Chin WX, Han Q, Ichiyama K, Lee CH, Eyo ZW, Ebina H, Takahashi H, Takahashi C, Tan BH, Hishiki T, Ohba K, Matsuyama T, Koyanagi Y, Tan YJ, Sawasaki T, Chu JJ, Vasudevan SG, Sano K, Yamamoto N. Characterization of RyDEN (C19orf66) as an Interferon-Stimulated Cellular Inhibitor against Dengue Virus Replication. PLoS Pathog. 2016 Jan 6;12(1):e1005357. doi: 10.1371/journal.ppat.1005357.
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竹田浩之、澤崎達也.  無細胞技術を基盤とした抗GPCRモノクローナル抗体作製技術の開発 Anti-GPR Mnocolonal Antibody Development based on Cell-free Technology. BioIndustry 2016 Jan. 33:56-62.
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2015

Matsunaga S, Masaoka T, Sawasaki T, Morishita R, Iwatani Y, Tatsumi M, Endo Y, Yamamoto N, Sugiura W, Ryo A. A cell-free enzymatic activity assay for the evaluation of HIV-1 drug resistance to protease inhibitors. Front Microbiol. 2015 Oct 31;6:1220. doi: 10.3389/fmicb.2015.01220.
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Ramadan A, Nemoto K, Seki M, Shinozaki K, Takeda H, Takahashi H, Sawasaki T. Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes. BMC Plant Biol. 2015 Nov 10;15(1):275. doi: 10.1186/s12870-015-0660-9.
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Furukawa H, Oka S, Shimada K, Masuo K, Nakajima F, Funano S, Tanaka Y, Komiya A, Fukui N, Sawasaki T, Tadokoro K, Nose M, Tsuchiya N, Tohma S. Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD. Biomark Insights. 2015 Aug 16;10:63-73. doi: 10.4137/BMI.S28209. LinkIconweb

Kaneko N, Ito Y, Iwasaki T, Takeda H, Sawasaki T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J. Reconstituted AIM2 inflammasome in cell-free system. J Immunol Methods. 2015 Aug 8. 426:76-81. doi: 10.1016/j.jim.2015.08.004. LinkIconweb

Ohnishi T, Yanazawa M, Sasahara T, Kitamura Y, Hiroaki H, Fukazawa Y, Kii I, Nishiyama T, Kakita A, Takeda H, Takeuchi A, Arai Y, Ito A, Komura H, Hirao H, Satomura K, Inoue M, Muramatsu SI, Matsui K, Tada M, Sato M, Saijo E, Shigemitsu Y, Sakai S, Umetsu Y, Goda N, Takino N, Takahashi H, Hagiwara M, Sawasaki T, Iwasaki G, Nakamura Y, Nabeshima YI, Teplow DB, Hoshi M. Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. Proc Natl Acad Sci U S A. 2015 Aug 11;112(32):E4465-74. doi: 10.1073/pnas.1421182112. LinkIconweb

Kinoshita-Kikuta E, Kinoshita E, Eguchi Y, Yanagihara S, Edahiro K, Inoue Y, Taniguchi M, Yoshida M, Yamamoto K, Takahashi H, Sawasaki T, Utsumi R, Koike T. Functional Characterization of the Receiver Domain for Phosphorelay Control in Hybrid Sensor Kinases. PLoS One. 2015 Jul 7;10(7):e0132598. doi: 10.1371/journal.pone.0132598.
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Nakajia M, Nagase S, Iida M, Takeda S, Yamashita M, Watari A, Shirasago Y, Fukasawa M, Takeda H, Sawasaki T, Yagi K, Kondoh M. Claudin-1 binder enhances epidermal permeability in a human keratinocyte model. J Pharmacol Exp Ther. 2015 Sep;354(3):440-7. doi: 10.1124/jpet.115.225391. LinkIconweb

Onishi S, Adnan E, Ishizaki J, Miyazaki T, Tanaka Y, Matsumoto T, Suemori K, Shudou M, Okura T, Takeda H, Sawasaki T, Yasukawa M, Hasegawa H. Novel Autoantigens Associated with Lupus Nephritis. PLoS One. 2015 Jun 22;10(6):e0126564. doi: 10.1371/journal.pone.0126564.LinkIconOpenAccess

Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T. Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay. Sci Rep. 2015 Jun 10;5:11333. doi: 10.1038/srep11333. LinkIconOpenAccessLinkIcon論文概要:創薬ターゲットGPCR〜 

Nemoto K, Takemori N, Seki M, Shinozaki K, Sawasaki T. Members of the Plant CRK-superfamily are Capable of trans-/auto-Phosphorylation of Tyrosine Residues. J Biol Chem. 2015 Jul 3;290(27):16665-77. doi: 10.1074/jbc.M114.617274. LinkIconwebLinkIcon論文概要:植物のチロシンキナーゼ〜 

Takemori N, Takemori A, Matsuoka K, Morishita R, Matsushita N, Aoshima M, Takeda H, Sawasaki T, Endo Y, Higashiyama S. High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system. Mol Biosyst. 2015 Feb;11(2):361-5. doi: 10.1039/c4mb00556b.LinkIconweb

James SJ, Jiao H, Teh HY, Takahashi H, Png CW, Phoon MC, Suzuki Y, Sawasaki T, Xiao H, Chow VT, Yamamoto N, Reynolds JM, Flavell RA, Dong C, Zhang Y. MAPK Phosphatase 5 Expression Induced by Influenza and Other RNA Virus Infection Negatively Regulates IRF3 Activation and Type I Interferon Response. Cell Rep. 2015 Mar 11. 10(10): 1722-34. doi: 10.1016/j.celrep.2015.02.030. LinkIconweb


竹田浩之、野澤 彰、澤崎達也. リポソーム添加型コムギ無細胞合成系による膜タンパク質合成とその利用. ファルマシア(日本薬学会) 2015 51(8):770-774.
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2014

Arimitsu E, Ogasawara T, Takeda H, Sawasaki T, Ikeda Y, Hiasa Y, Maeyama K. The ligand binding ability of dopamine D1 receptors synthesized using a wheat germ cell-free protein synthesis system with liposomes. Eur J Pharmacol. 2014 Dec 15;745:117-22. LinkIconweb

Sakamaki K, Shimizu K, Iwata H, Imai K, Satou Y, Funayama N, Nozaki M, Yajima M, Nishimura O, Higuchi M, Chiba K, Yoshimoto M, Kimura H, Gracey AY, Shimizu T, Tomii K, Goto O, Akasaka K, Sawasaki T, Miller DJ. The apoptotic initiator caspase-8: its functional ubiquity and genetic diversity during animal evolution. Mol Biol Evol. 2014 Dec;31(12):3282-301. LinkIconweb

Mitsunami T, Nishihara M, Galis I, Alamgir KM, Hojo Y, Fujita K, Sasaki N, Nemoto K, Sawasaki T, Arimura GI. Overexpression of the PAP1 Transcription Factor Reveals a Complex Regulation of Flavonoid and Phenylpropanoid Metabolism in Nicotiana tabacum Plants Attacked by Spodoptera litura. PLoS One. 2014 Sep 30;9(9):e108849. LinkIconOpenAccess

Tan BH, Suzuki Y, Takahashi H, Ying PH, Takahashi C, Han Q, Chin WX, Chao SH, Sawasaki T, Yamamoto N, Suzuki Y. Identification of RFPL3 Protein as a Novel E3 Ubiquitin Ligase Modulating the Integration Activity of Human Immunodeficiency Virus, Type 1 Preintegration Complex Using a Microtiter Plate-based Assay. J Biol Chem. 2014 Sep 19;289(38):26368-82. LinkIconweb

Fu SC, Imai K, Sawasaki T, Tomii K. ScreenCap3: improving prediction of caspase-3 cleavage sites using experimentally verified non-cleavage sites. Proteomics. 2014 Sep;14(17-18):2042-6. LinkIconweb

Shimizu K, Uematsu A, Imai Y, Sawasaki T. Pctaire1/Cdk16 promotes skeletal myogenesis by inducing myoblast migration and fusion. FEBS Lett. 2014 Aug 25;588(17):3030-7. LinkIconweb

Masaki T, Matsunaga S, Takahashi H, Nakashima K, Kimura Y, Ito M, Matsuda M, Murayama A, Kato T, Hirano H, Endo Y, Lemon SM, Wakita T, Sawasaki T, Suzuki T. Involvement of Hepatitis C Virus NS5A Hyperphosphorylation Mediated by Casein Kinase I-α in Infectious Virus Production. J Virol. 2014 Jul 1;88(13):7541-7555. LinkIconweb

Mizutani Y, Tsuge S, Takeda H, Hasegawa Y, Shiogama K, Onouchi T, Inada KI, Sawasaki T, Tsutsumi Y. In situ visualization of plasma cells producing antibodies reactive to Porphyromonas gingivalis in periodontitis: The application of the enzyme-labeled antigen method. Mol Oral Microbiol. 2014 Aug;29(4):156-73. LinkIconweb

Nagayoshi Y, Nakamura M, Matsuoka K, Ohtsuka T, Mori Y, Kono H, Aso T, Ideno N, Takahata S, Ryo A, Takeda H, Ito T, Oda Y, Endo Y, Sawasaki T, Tanaka M. Profiling of Autoantibodies in Sera of Pancreatic Cancer Patients. Ann Surg Oncol. 2014 Jun;21 Suppl 3:S459-65. LinkIconweb

Takiuchi T, Nakagawa T, Tamiya H, Fujita H, Sasaki Y, Saeki Y, Takeda H, Sawasaki T, Buchberger A, Kimura T, Iwai K. Suppression of LUBAC-mediated linear ubiquitination by a specific interaction between LUBAC and the deubiquitinases CYLD and OTULIN. Genes Cells. 2014 Mar;19(3):254-272. LinkIconweb

Kudoh A, Takahama S, Sawasaki T, Ode H, Yokoyama M, Okayama A, Ishikawa A, Miyakawa K, Matsunaga S, Kimura H, Sugiura W, Sato H, Hirano H, Ohno S, Yamamoto N, Ryo A. The phosphorylation of HIV-1 Gag by atypical protein kinase C facilitates viral infectivity by promoting Vpr incorporation into virions. Retrovirology. 2014 Jan 22;11:9. LinkIconweb

Ito M, Takahashi H, Sawasaki T, Ohnishi K, Hikichi Y, Kiba A. Novel type of adenylyl cyclase participates in tabtoxinine-β-lactam-induced cell death and occurrence of wildfire disease in Nicotiana benthamiana. Plant Signal Behav. 2014 Jan 7;8(12). pii: e27420. LinkIconweb

2013

Iwasaki T, Katayama T, Kohama K, Endo Y, Sawasaki T. Myosin phosphatase is inactivated by caspase-3 cleavage and phosphorylation of myosin phosphatase targeting subunit 1 during apoptosis. Mol Biol Cell. 2013 Mar;24(6):748-56. LinkIconwebLinkIcon論文概要:新しいミオシンII〜 

Mizutani Y, Matsuoka K, Takeda H, Shiogama K, Inada KI, Hayakawa K, Yamada H, Miyazaki T, Sawasaki T, Endo Y, Tsutsumi Y.Novel approach to identifying autoantibodies in rheumatoid synovitis with a biotinylated human autoantigen library and the enzyme-labeled antigen method. J Immunol Methods. 2013 Jan 31;387(1-2):57-70. LinkIconweb

Ishigami T, Abe K, Aoki I, Minegishi S, Ryo A, Matsunaga S, Matsuoka K, Takeda H, Sawasaki T, Umemura S, Endo Y. Anti-interleukin-5 and multiple autoantibodies are associated with human atherosclerotic diseases and serum interleukin-5 levels. FASEB J. 2013 Sep;27(9):3437-45. LinkIconweb

Shimizu K, Sawasaki T. Nek5, a novel substrate for caspase-3, promotes skeletal muscle differentiation by up-regulating caspase activity. FEBS Lett. 2013 Jul 11;587(14):2219-25. LinkIconweb

Germain H, Gray-Mitsumune M, Houde J, Benhamman R, Sawasaki T, Endo Y, Matton DP. The Solanum chacoense ovary receptor kinase 11 (ScORK11) undergoes tissue-dependent transcriptional, translational and post-translational regulation. Plant Physiol Biochem. 2013 Sep;70:261-8. LinkIconweb

Nakano M, Nishihara M, Yoshioka H, Takahashi H, Sawasaki T, Ohnishi K, Hikichi Y, Kiba A. Suppression of DS1 Phosphatidic Acid Phosphatase Confirms Resistance to Ralstonia solanacearum in Nicotiana benthamiana. PLoS One. 2013 Sep 20;8(9):e75124. LinkIconOpenAccess

2012

Kominami K, Nagai T, Sawasaki T, Tsujimura Y, Yashima K, Sunaga Y, Tsuchimochi M, Nishimura J, Chiba K, Nakabayashi J, Koyamada K, Endo Y, Yokota H, Miyawaki A, Manabe N, Sakamaki K. In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis. PLoS One. 2012;7(11):e50218.LinkIconOpenAccess

Miyakawa K, Sawasaki T, Matsunaga S, Tokarev A, Quinn G, Kimura H, Nomaguchi M, Adachi A, Yamamoto N, Guatelli J, Ryo A. Interferon-Induced SCYL2 Limits Release of HIV-1 by Triggering PP2A-Mediated Dephosphorylation of the Viral Protein Vpu. Sci Signal. 2012 Oct 9;5(245):ra73. LinkIconweb

Takahashi H, Takahashi C, Moreland NJ, Chang YT, Sawasaki T, Ryo A, Vasudevan SG, Suzuki Y, Yamamoto N. Establishment of a robust dengue virus NS3-NS5 binding assay for identification of protein-protein interaction inhibitors. Antiviral Res. 2012 Oct 13;96(3):305-314. LinkIconweb

Shimizu K, Takahama S, Endo Y, Sawasaki T. Stress-Inducible Caspase Substrate TRB3 Promotes Nuclear Translocation of Procaspase-3. PLoS One. 2012;7(8):e42721. LinkIconOpenAccess

Takahashi H, Ozawa A, Nemoto K, Nozawa A, Seki M, Shinozaki K, Takeda H, Endo Y, Sawasaki T. Genome-wide biochemical analysis of Arabidopsis protein phosphatase using a wheat cell-free system. FEBS Lett. 2012 Sep 21;586(19):3134-41. LinkIconweb

Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K. The molecular mechanism of apoptosis upon caspase-8 activation: Quantitative experimental validation of a mathematical model. Biochim Biophys Acta. 2012 Aug 3;75(15):4863-73. LinkIconweb

Matsunaga S, Sawasaki T, Ode H, Morishita R, Furukawa A, Sakuma R, Sugiura W, Sato H, Katahira M, Takaori-Kondo A, Yamamoto N, Ryo A. Molecular and enzymatic characterization of XMRV protease by a cell-free proteolytic analysis. J Proteomics. 2012 Aug 3;75(15):4863-73. LinkIconweb

Kiba A, Nakano M, Vincent-Pope P, Takahashi H, Sawasaki T, Endo Y, Ohnishi K, Yoshioka H, Hikichi Y. A novel Sec14 phospholipid transfer protein from Nicotiana benthamiana is up-regulated in response to Ralstonia solanacearum infection, pathogen associated molecular patterns and effector molecules and involved in plant immunity. J Plant Physiol. 2012 Jul 1;169(10):1017-22. LinkIconweb

2011


Tsuge S, Mizutani Y, Matsuoka K, Sawasaki T, Endo Y, Naruishi K, Maeda H, Takashiba S, Shiogama K, Inada KI, Tsutsumi Y. Specific In Situ Visualization of Plasma Cells Producing Antibodies Against Porphyromonas Gingivalis in Gingival Radicular Cyst: Application of the Enzyme-Labeled Antigen Method. J Histochem Cytochem. 2011 Jul;59(7):673-89. LinkIconweb

Akagi T, Shimizu K, Takahama S, Iwasaki T, Sakamaki K, Endo Y, Sawasaki T. Caspase-8 cleavage of the interleukin-21 (IL-21) receptor is a negative feedback regulator of IL-21 signaling. FEBS Lett. 2011 Jun 23;585(12):1835-40. LinkIconweb

Nemoto K, Seto T, Takahashi H, Nozawa A, Seki M, Shinozaki K, Endo Y, Sawasaki T. Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system. Phytochemistry. 2011 Apr 6 Jul;72(10):1136-44. LinkIconweb

Nozawa A, Ogasawara T, Matsunaga S, Iwasaki T, Sawasaki T, Endo Y. Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system. BMC Biotechnol. 2011 Apr 11;11:35. LinkIconOpenAccess

Makino S, Sawasaki T, Endo Y, Takai K. Use of domain enzymes from wheat RNA ligase for in vitro preparation of RNA molecules. Biochem Biophys Res Commun. 2011 Jan 28;404(4):1050-4. LinkIconweb

Madono M, Sawasaki T, Morishita R, Endo Y. Wheat germ cell-free protein production system for post-genomic research. N Biotechnol. 2011 Apr 30;28(3):211-7. LinkIconweb

2010

Tadokoro D, Takahama S, Shimizu K, Hayashi S, Endo Y, Sawasaki T. Characterization of a caspase-3-substrate kinome using an N- and C-terminally tagged protein kinase library produced by a cell-free system. Cell Death & Dis. 2010 Oct 28;1:e84. LinkIconweb

Arimura GI, Sawasaki T. Arabidopsis CPK3 plays extensive roles in various biological and environmental responses. Plant Signal Behav. 2010 Oct 7;5(10). LinkIconweb

Matsuoka K, Komori H, Nose M, Endo Y, Sawasaki T. Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis. J Proteome Res. 2010 Aug 6;9(8):4264-73. LinkIconweb

Makino SI, Sawasaki T, Endo Y, Takai K. In vitro dissection revealed that the kinase domain of wheat RNA ligase is physically isolatable from the flanking domains as a non-overlapping domain enzyme. Biochem Biophys Res Commun. 2010 Jul 9;397(4):762-6. LinkIconweb

Matsunaga S, Matsuoka K, Shimizu K, Endo Y, Sawasaki T. Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins. BMC Biotechnol. 2010 Jun 4;10(1):42. LinkIconOpenAccess

Kanchiswamy CN, Takahashi H, Quadro S, Maffei ME, Bossi S, Bertea C, Zebelo SA, Muroi A, Ishihama N, Yoshioka H, Boland W, Takabayashi J, Endo Y, Sawasaki T, Arimura G. Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling. BMC Plant Biol. 2010 May 26;10:97. LinkIconOpenAccess

Takai K, Sawasaki T, Endo Y. The wheat-germ cell-free expression system. Curr Pharm Biotechnol. 2010 Apr;11(3):272-8. LinkIconweb

Tanaka Y, Komori H, Mori S, Soga Y, Tsubaki T, Terada M, Miyazaki T, Fujino T, Nakamura S, Kanno H, Sawasaki T, Endo Y, Nose M. Evaluating the role of rheumatoid factors for the development of rheumatoid arthritis in a mouse model with a newly established ELISA system. Tohoku J Exp Med. 2010;220(3):199-206. LinkIconweb

Takai K, Sawasaki T, Endo Y. Practical cell-free protein synthesis system using purified wheat embryos. Nat Protoc. 2010;5(2):227-238. LinkIconweb

Sawasaki T, Endo Y. Cell-Free-Based Protein Microarray Technology Using Agarose/DNA Microplate. Methods Mol Biol. 2010;607:63-72. LinkIconweb

Tsuboi T, Takeo S, Sawasaki T, Torii M, Endo Y. An efficient approach to the production of vaccines against the malaria parasite. Methods Mol Biol. 2010;607:73-83. LinkIconweb

Watanabe M, Miyazono K, Tanokura M, Sawasaki T, Endo Y, Kobayashi I. Cell-Free Protein Synthesis for Structure Determination by X-ray Crystallography.Methods Mol Biol. 2010;607:149-160. LinkIconweb

1999-2009

Shimada H, Hirai KI, Simamura E, Hatta T, Iwakiri H, Mizuki K, Hatta T, Sawasaki T, Matsunaga S, Endo Y, Shimizu S. Paraquat Toxicity Induced by Voltage-dependent Anion Channel 1 Acts as an NADH-dependent Oxidoreductase. J Biol Chem. 2009 Oct 16;284(42):28642-28649. LinkIconweb

Igawa T, Fujiwara M, Takahashi H, Sawasaki T, Endo Y, Seki M, Shinozaki K, Fukao Y, Yanagawa Y. Isolation and identification of ubiquitin-related proteins from Arabidopsis seedlings. J Exp Bot. 2009 60(11):3067-73. LinkIconweb

Nozawa A, Matsubara Y, Tanaka Y, Takahashi H, Akagi T, Seki M, Shinozaki K, Endo Y, Sawasaki T. Construction of a protein library of Arabidopsis transcription factors using wheat cell-free protein production system and its application for DNA binding analysis. Biosci Biotechnol Biochem. 2009 Jul;73(7):1661-4. LinkIconweb

Takahashi H, Nozawa A, Seki M, Shinozaki K, Endo Y Sawasaki T. A Simple and High-sensitivity Method for Analysis of Ubiquitination and Polyubiquitination Based on Wheat Cell-free Protein Synthesis. BMC Plant Biology. 2009 Apr 6;9:39. LinkIconOpenAccess

Kobayashi T, Kodani Y, Nozawa A, Endo Y, Sawasaki T. DNA-binding profiling of human hormone nuclear receptors via fluorescence correlation spectroscopy in a cell-free system. FEBS Lett. 2008 Aug 6;582(18):2737-44. LinkIconweb


Masaoka T, Nishi M, Ryo A, Endo Y, Sawasaki T. The wheat germ cell-free based screening of protein substrates of calcium/calmodulin-dependent protein kinase II delta. FEBS Lett. 2008 Jun 11;582(13):1795-801. LinkIconweb

Sawasaki T, Nishihara M, Endo Y. RIP and RALyase cleave the sarcin/ricin domain, a critical domain for ribosome function, during senescence of wheat coleoptiles. Biochem Biophys Res Commun. 2008 Jun 13;370(4):561-5. LinkIconweb

Bardóczy V, Géczi V, Sawasaki T, Endo Y, Mészáros T. A set of ligation-independent in vitro translation vectors for eukaryotic protein production. BMC Biotechnol. 2008 Mar 27;8:32. LinkIconOpenAccess

Tsuboi T, Takeo S, Iriko H, Jin L, Tsuchimochi M, Matsuda S, Han ET, Otsuki H, Kaneko O, Sattabongkot J, Udomsangpetch R, Sawasaki T, Torii M, Endo Y. Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. Infect Immun. 2008 Apr;76(4):1702-8. LinkIconweb

Sawasaki T, Kamura N, Matsunaga S, Saeki M, Tsuchimochi M, Morishita R, Endo Y. Arabidopsis HY5 protein functions as a DNA-binding tag for purification and functional immobilization of proteins on agarose/DNA microplate. FEBS Lett. 2008 Jan 23;582(2):221-8. LinkIconweb

Germain H, Houde J, Gray-Mitsumune M, Sawasaki T, Endo Y, Rivoal J, Matton DP. Characterization of ScORK28, a transmembrane functional protein receptor kinase predominantly expressed in ovaries from the wild potato species Solanum chacoense. FEBS Lett. 2007 Oct 30;581(26):5137-42. LinkIconweb

Sawasaki T, Morishita R, Gouda MD, Endo Y. Methods for high-throughput materialization of genetic information based on wheat germ cell-free expression system. Methods Mol Biol. 2007;375:95-106. Review. LinkIconweb

Endoh T, Mie M, Funabashi H, Sawasaki T, Endo Y, Kobatake E. Construction of intramolecular luciferase complementation probe for detecting specific RNA. Bioconjug Chem. 2007 May-Jun;18(3):956-62. LinkIconweb

Abe M, Ohno S, Yokogawa T, Nakanishi T, Arisaka F, Hosoya T, Hiramatsu T, Suzuki M, Ogasawara T, Sawasaki T, Nishikawa K, Kitamura M, Hori H, Endo Y. Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing. Proteins. 2007 May 15;67(3):643-52. LinkIconweb

Miyazono K, Watanabe M, Kosinski J, Ishikawa K, Kamo M, Sawasaki T, Nagata K, Bujnicki JM, Endo Y, Tanokura M, Kobayashi I. Novel protein fold discovered in the PabI family of restriction enzymes. Nucleic Acids Res. 2007;35(6):1908-18. LinkIconweb

Makino S, Sawasaki T, Tozawa Y, Endo Y, Takai K. Covalent circularization of exogenous RNA during incubation with a wheat embryo cell extract. Biochem Biophys Res Commun. 2006 Sep 8;347(4):1080-7. LinkIconweb

Endo Y, Sawasaki T. Cell-free expression systems for eukaryotic protein production. Curr Opin Biotechnol. 2006 Aug;17(4):373-80. Review. LinkIconweb

Kominami K, Takagi C, Kurata T, Kitayama A, Nozaki M, Sawasaki T, Kuida K, Endo Y, Manabe N, Ueno N, Sakamaki K. The initiator caspase, caspase-10beta, and the BH-3-only molecule, Bid, demonstrate evolutionary conservation in Xenopus of their pro-apoptotic activities in the extrinsic and intrinsic pathways. Genes Cells. 2006 Jul;11(7):701-17. LinkIconweb

Ihara H, Mie M, Funabashi H, Takahashi F, Sawasaki T, Endo Y, Kobatake E. In vitro selection of zinc finger DNA-binding proteins through ribosome display. Biochem Biophys Res Commun. 2006 Jul 7;345(3):1149-54. LinkIconweb

Hirano N, Sawasaki T, Tozawa Y, Endo Y, Takai K. Tolerance for random recombination of domains in prokaryotic and eukaryotic translation systems: Limited interdomain misfolding in a eukaryotic translation system. Proteins. 2006 Aug 1;64(2):343-54. LinkIconweb

Endo Y, Sawasaki T. Advances in genome-wide protein expression using the wheat germ cell-free system. Methods Mol Biol. 2005;310:145-67. LinkIconweb

Sawasaki T, Gouda MD, Kawasaki T, Tsuboi T, Tozawa Y, Takai K, Endo Y. The wheat germ cell-free expression system: methods for high-throughput materialization of genetic information. Methods Mol Biol. 2005;310:131-44. LinkIconweb

Kamura N, Sawasaki T, Kasahara Y, Takai K, Endo Y. Selection of 5'-untranslated sequences that enhance initiation of translation in a cell-free protein synthesis system from wheat embryos. Bioorg Med Chem Lett. 2005 Dec 15;15(24):5402-6. LinkIconweb

Takahashi F, Funabashi H, Mie M, Endo Y, Sawasaki T, Aizawa M, Kobatake E. Activity-based in vitro selection of T4 DNA ligase. Biochem Biophys Res Commun. 2005 Oct 28;336(3):987-93. LinkIconweb

Miyazaki T, Ono M, Qu WM, Zhang MC, Mori S, Nakatsuru S, Nakamura Y, Sawasaki T, Endo Y, Nose M. Implication of allelic polymorphism of osteopontin in the development of lupus nephritis in MRL/lpr mice. Eur J Immunol. 2005 May;35(5):1510-20. LinkIconweb

Kobayashi T, Okamoto N, Sawasaki T, Endo Y. Detection of protein-DNA interactions in crude cellular extracts by fluorescence correlation spectroscopy. Anal Biochem. 2004 Sep 1;332(1):58-66. LinkIconweb

Sawasaki T, Hasegawa Y, Morishita R, Seki M, Shinozaki K, Endo Y. Genome-scale, biochemical annotation method based on the wheat germ cell-free protein synthesis system. Phytochemistry. 2004 Jun;65(11):1549-55. LinkIconweb

Endo Y, Sawasaki T. High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system. J Struct Funct Genomics. 2004;5(1-2):45-57. LinkIconweb

Madin K, Sawasaki T, Kamura N, Takai K, Ogasawara T, Yazaki K, Takei T, Miura K, Endo Y. Formation of circular polyribosomes in wheat germ cell-free protein synthesis system. FEBS Lett. 2004 Mar 26;562(1-3):155-9. LinkIconweb

Ozawa A, Sawasaki T, Takai K, Uchiumi T, Hori H, Endo Y. RALyase; a terminator of elongation function of depurinated ribosomes. FEBS Lett. 2003 Dec 18;555(3):455-8. LinkIconweb

Kawasaki T, Gouda MD, Sawasaki T, Takai K, Endo Y. Efficient synthesis of a disulfide-containing protein through a batch cell-free system from wheat germ. Eur J Biochem. 2003 Dec;270(23):4780-6. LinkIconweb

Endo Y, Sawasaki T. High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system. Biotechnol Adv. 2003 Nov;21(8):695-713. Review. LinkIconweb

Miyamoto-Sato E, Takashima H, Fuse S, Sue K, Ishizaka M, Tateyama S, Horisawa K, Sawasaki T, Endo Y, Yanagawa H. Highly stable and efficient mRNA templates for mRNA-protein fusions and C-terminally labeled proteins. Nucleic Acids Res. 2003 Aug 1;31(15):e78. LinkIconweb

Morita EH, Sawasaki T, Tanaka R, Endo Y, Kohno T. A wheat germ cell-free system is a novel way to screen protein folding and function. Protein Sci. 2003 Jun;12(6):1216-21. web

Hori H, Kubota S, Watanabe K, Kim JM, Ogasawara T, Sawasaki T, Endo Y. Aquifex aeolicus tRNA (Gm18) methyltransferase has unique substrate specificity. TRNA recognition mechanism of the enzyme. J Biol Chem. 2003 Jul 4;278(27):25081-90. Epub 2003 Apr 18. LinkIconweb

Ito Y, Ozawa A, Sawasaki T, Endo Y, Ochi K, Tozawa Y. OsRALyase1, a putative F-box protein identified in rice, Oryza sativa, with enzyme activity identical to that of wheat RALyase. Biosci Biotechnol Biochem. 2002 Dec;66(12):2727-31. LinkIconweb

Sawasaki T, Ogasawara T, Morishita R, Endo Y. A cell-free protein synthesis system for high-throughput proteomics. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14652-7. LinkIconweb

Sawasaki T, Hasegawa Y, Tsuchimochi M, Kamura N, Ogasawara T, Kuroita T, Endo Y. A bilayer cell-free protein synthesis system for high-throughput screening of gene products. FEBS Lett. 2002 Mar 6;514(1):102-5. LinkIconweb

Doi N, Takashima H, Kinjo M, Sakata K, Kawahashi Y, Oishi Y, Oyama R, Miyamoto-Sato E, Sawasaki T, Endo Y, Yanagawa H. Novel fluorescence labeling and high-throughput assay technologies for in vitro analysis of protein interactions.
Genome Res. 2002 Mar;12(3):487-92. LinkIconweb

Sawasaki T, Oka T, Endo Y. Liver perchloric acid-soluble ribonuclease. Methods Enzymol. 2001;342:44-54. LinkIconweb

Madin K, Sawasaki T, Ogasawara T, Endo Y. A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes. Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):559-64. LinkIconweb

Ogasawara T, Sawasaki T, Morishita R, Ozawa A, Madin K, Endo Y. A new class of enzyme acting on damaged ribosomes: ribosomal RNA apurinic site specific lyase found in wheat germ. EMBO J. 1999 Nov 15;18(22):6522-31. LinkIconweb

Morishita R, Kawagoshi A, Sawasaki T, Madin K, Ogasawara T, Oka T, Endo Y. Ribonuclease activity of rat liver perchloric acid-soluble protein, a potent inhibitor of protein synthesis. J Biol Chem. 1999 Jul 16;274(29):20688-92. LinkIconweb

日本語総説

2002-

野澤彰, 戸澤譲, 澤崎達也, 遠藤弥重太 “IIターゲットタンパク研究プログラムの紹介 1.生産部門 無細胞蛋白質合成と非天然型アミノ酸部位特異的導入を特徴とする高難度蛋白質試料調整技術の開発 コムギ胚芽無細胞系を用いた膜蛋白質生産”『蛋白質 核酸 酵素』融合発展する構造生物学とケミカルバイオロジーの最前線, Vol.54 No.12, 1443-1447,共立出版 (2009) 9月

遠藤弥重太, 高井和幸, 澤崎達也 “コムギ胚芽由来無細胞タンパク質合成” やさしい原理からはいるタンパク質科学実験法 1:タンパク質をつくる‐抽出・精製と合成, 141-148, 株式会社化学同人(2008)12月

高井和幸,澤崎達也,遠藤弥重太 “コムギ胚芽抽出液を用いた翻訳” 無敵のバイオテクニカルシリーズ「RNA実験ノート 上 RNAの基本的な取り扱いから解析手法まで」, 138-142, 羊土社(2008)3月

遠藤弥重太”序論:無細胞生命科学の提案” 生化学,Vol.79 No.3,203-204,社団法人日本生化学会(2007)3月

遠藤弥重太,澤崎達也”コムギ無細胞タンパク質合成法” 生化学,Vol.79 No.3,229-238,社団法人日本生化学会(2007)3月

澤崎達也,遠藤弥重太”無細胞タンパク質合成法を用いたハイスループットタンパク質機能解析法の開発とタンパク質生物学分野開拓に向けた応用” 生化学,Vol.79 No.3,278-286,社団法人日本生化学会(2007)3月

能勢眞人,小森浩章,宮崎龍彦,澤崎達也,遠藤弥重太”膠原病のゲノム解析に基づく疾病関連タンパク質の探索ー無細胞タンパク質合成系の応用ー” 生化学,Vol.79 No.3,287-295,社団法人日本生化学会(2007)3月

澤崎達也、竹田浩之、高橋宏隆、根本圭一郎, 無細胞タンパク質アレイを用いたプロテインキナーゼおよびユビキチンリガーゼの基質タンパク質探索技術、生化学、2013, Vol 85, 438-446

澤崎達也、遠藤弥重太 n vitroトランスレーションシステム  細胞工学別冊 細胞工学シリーズ21 改訂3版 モデル植物の実験プロトコール -イネ・シロイヌナズナ・ミヤコグサ編- 監修 島本功・岡田清孝・田畑哲之, 230-233,株式会社秀潤社(2005)

澤崎達也、遠藤弥重太  無細胞蛋白質合成系:コムギ胚芽系 『蛋白質核酸酵素』増刊 バイオ高性能機器・新技術利用マニュアル, 49巻No.11, 1514-1519, 共立出版株式会社 (2004)

細胞を使わずにタンパク質をつくる 遠藤弥重太、澤崎達也『高分子』特集=高分子とバイオテクノロジー,53巻11月号, 868-871 (2004)

遠藤弥重太、澤崎達也  無細胞タンパク質合成システム  ゲノミクス・プロテオミクスの新展開 生物情報の解析と応用 監修・今中忠行,573-577,第2編・第2章・第1節,株式会社エヌ・ティー・エス(2004)

遠藤弥重太、澤崎達也  無細胞タンパク質合成技術を基盤としたハイスループット機能・構造解析 第1特集 タンパク質を攻略する!発現システムと機能解析の最新テクノロジー Bioベンチャー 9-10月号(Vol.3 No.5),22-24,羊土社(2003)

遠藤弥重太、澤崎達也  無細胞蛋白質合成法の進歩 ポストゲノム科学への応用 VI. RNAバイオテクノロジー 蛋白質核酸酵素、48巻No.4, 549-554,共立出版株式会社 (2003)

澤崎達也、遠藤弥重太 コムギ胚芽無細胞タンパク質合成法 テクノ・トレンド、 Bioベンチャー 5-6月号(Vol.3 No.3), 48-49,羊土社 (2003)

澤崎達也、関原明、篠崎一雄、遠藤弥重太 植物cDNAからの無細胞蛋白質合成 −コムギ胚芽系を用いたシロイヌナズナ蛋白質の網羅的発現− 蛋白質核酸酵素増刊 構造プロテオミクス, 47巻 No.8, 1003-1008, 共立出版株式会社(2002)

遠藤弥重太、澤崎達也 コムギ胚芽から調製した高効率無細胞タンパク質合成システム テクニカルノート 生化学 第74巻第4号, 326-330 (2002)

特許申請中(公開済み)

2002-

遠藤弥重太、タンパク質合成・回収一体型無細胞タンパク質合成方法、公開番号:2007-195545 (H19.8.9)

遠藤弥重太・坪井敬文・奥山正明・大瀬戸光明、疹ウイルスタンパク質の製造方法、公開番号:2007-191477 (H19.8.2)

遠藤弥重太・澤崎達也、タンパク質チップ作製用試薬、国際公開番号:WO2005/015212(H17.2.17)

遠藤弥重太・河野俊之、アミノ酸選択的標識化蛋白質合成方法、国際公開番号:WO2005/010195(H17.2.3)

遠藤弥重太・澤崎達也・小笠原富夫・森下了・佐伯美帆呂・佐藤智久・北本綾、ハイスループット合成システム及び該システムを自動で行うための合成装置、国際公開番号:WO2004/097014(H16.11.11)

遠藤弥重太・澤崎達也、jidou 蛋白質合成方法及びそれを行うための装置、国際公開番号:WO2004/070047(H16.8.19)

遠藤弥重太、汎用性および高効率機能を備えた鋳型分子およびこれを利用する無細胞タンパク質合成手段、国際公開番号:WO01/027260(H13.4.19)

川崎平康・澤崎達也・遠藤弥重太 標識化単鎖抗体およびその利用 公開番号;WO04/009639

遠藤弥重太・澤崎達也  自動蛋白質合成方法及びそれを行うための装置 公開番号;WO04/070047

遠藤弥重太・澤崎達也・小笠原富夫・森下了・佐伯美幌呂・佐藤智久・北本綾  無細胞系合成システム 公開番号;WO04/097014

澤崎達也・小笠原富夫・遠藤弥重太 タンパク質合成方法 公開番号;2003-047496

遠藤弥重太・澤崎達也 翻訳効率制御活性を有するヌクレオチド配列及びその利用 特許登録; 特許第3701292号

遠藤弥重太・川崎平康・澤崎達也 無細胞タンパク質合成反応液、その調整方法及びそれを用いたタンパク質合成方法 公開番号;WO03/072796

遠藤弥重太・澤崎達也・小笠原富夫 無細胞タンパク質合成方法 米国特許取得:平成17年3月  特許番号686977 日本特許登録;平成18年2月 特許第3768190号