発表論文リスト

Kazuhiro Sakamaki, Naohisa Sakamoto, Yuki Tsujimura, Takahiro Iwasaki, Takuma Kawamura, Jun Nakabayashi, Rhea S. D’Souza, Arooma Jannat, Ken-ichiro Takeshima, Hiroyuki Takeda, Koji Koyamada, Hideo Yokota
Caspase-mediated cleavage of a scaffold protein, MPRIP, yields a truncated form that is involved in repetitive bleb formation.
FEBS Journal 2025
doi: 10.1111/febs.17422　 PMID: NA

Tachibana K, Hirayama R, Sato N, Hattori K, Kato T, Takeda H, Kondoh M*.
Association of Plasma Claudin-5 with Age and Alzheimer Disease.
Int J Mol Sci. 2024 Jan 24;25(3):1419. Open access
doi: 10.3390/ijms25031419. PMID: 38338697

Ueda R, Hashimoto R, Fujii Y, Menezes JCJMDS, Takahashi H, Takeda H, Sawasaki T, Motokawa T, Tokunaga K, Fujita H*.
Membrane-Associated Ubiquitin Ligase RING Finger Protein 152 Orchestrates Melanogenesis via Tyrosinase Ubiquitination.
Membranes (Basel). 2024 Feb 1;14(2):43. Open access
doi: 10.3390/membranes14020043. PMID: 38392670

Miyata A, Chisuga T, Kambe A, Miyata R, Kawamura Y, Takeda H*, Ito S*, Nakano S*
Design of Ancestral Sortase E that is Applicable in Protein Biomaterial Synthesis
ACS Catalysis, 3514-3523, (2024). High Impact Factor Journal
doi:10.1021/acscatal.4c00487.

Kaneko, N., Kurata, M., Yamamoto, T., Sakamoto, A., Takada, Y., Kosako, H., Takeda, H., Sawasaki, T., & Masumoto, J.
CANE, a Component of the NLRP3 Inflammasome, Promotes Inflammasome Activation.
Journal of immunology (Baltimore, Md. : 1950), 213(1), 86–95. (2024).
PMID: 38787200 DOI: 10.4049/jimmunol.2300175

Kazuyuki Sato, Satoki Teranishi, Atsushi Sakaue, Yukiko Karuo, Atsushi Tarui, Kentaro Kawai, Hiroyuki Takeda, Tatsuo Kinashi and Masaaki Omote
Rhodium-catalyzed homo-coupling reaction of aryl Grignard reagents and its application for the synthesis of an integrin inhibitor
Beilstein J. Org. Chem. 2024, 20, 1341–1347. Open access
doi: https://doi.org/10.3762/bjoc.20.118

Takeda H*, Ozawa T, Zenke H, Ohnuki Y, Umeda Y, Zhou W, Tomoda H, Takechi A, Narita K, Shimizu T, Miyakawa T, Ito Y, Sawasaki T. VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica).
Front Bioeng Biotechnol. 2023 Sep 12;11:1265582. Open access
doi: 10.3389/fbioe.2023.1265582. PMID: 37771574

Nakada-Masuta T, Takeda H, Uchida K.
Novel Approach for Obtaining Variable Domain of New Antigen Receptor with Different Physicochemical Properties from Japanese Topeshark (Hemitriakis japanica).
Mar Drugs. 2023 Oct 24;21(11):550. Open access
doi: 10.3390/md21110550. PMID: 37999374

Han SI, Nakakuki M, Nakagawa Y, Wang Y, Araki M, Yamamoto Y, Tokiwa H, Takeda H, Mizunoe Y, Motomura K, Ohno H, Kainoh K, Murayama Y, Aita Y, Takeuchi Y, Osaki Y, Miyamoto T, Sekiya M, Matsuzaka T, Yahagi N, Sone H, Daitoku H, Sato R, Kawano H, Shimano H.
Rhomboid protease RHBDL4/RHBDD1 cleaves SREBP-1c at endoplasmic reticulum monitoring and regulating fatty acids.
PNAS Nexus. 2023 Nov 8;2(11):pgad351. Open access
doi: 10.1093/pnasnexus/pgad351. PMID: 37954160

Fujiwara K, Maekawa M, Iimori Y, Ogawa A, Urano T, Kono N, Takeda H, Higashiyama S, Arita M, Murai J. The crucial role of single-stranded DNA binding in enhancing sensitivity to DNA-damaging agents for Schlafen 11 and Schlafen 13.
iScience. 2023 Nov 23;26(12):108529. Open access
doi: 10.1016/j.isci.2023.108529. PMID: 38125019

Nishiguchi R, Tanaka T, Hayashida J, Nakagita T, Zhou W, Takeda H.
Evaluation of Cell-Free Synthesized Human Channel Proteins for In Vitro Channel Research.
Membranes (Basel). 2022 Dec 30;13(1):48. Open access
doi: 10.3390/membranes13010048. PMID: 36676855

Morita M, Kanoi BN, Shinzawa N, Kubota R, Takeda H, Sawasaki T, Tsuboi T, Takashima E.
AGIA Tag System for Ultrastructural Protein Localization Analysis in Blood-Stage Plasmodium falciparum.
Front Cell Infect Microbiol. 2021 Dec 15;11:777291. Open access
doi: 10.3389/fcimb.2021.777291. PMID: 34976861

Segawa R, Takeda H, Yokoyama T, Ishida M, Miyata C, Saito T, Ishihara R, Nakagita T, Sasano Y, Kanoh N, Iwabuchi Y, Mizuguchi M, Hiratsuka M, Hirasawa N.
A chalcone derivative suppresses TSLP induction in mice and human keratinocytes through binding to BET family proteins.
Biochem Pharmacol. 2021 Dec;194:114819.
doi: 10.1016/j.bcp.2021.114819. PMID: 34757034.
 
Ishigami T, Nanki T, Sugawara T, Uchida K, Takeda H, Sawasaki T, Chen L, Doi H, Arakawa K, Saigo S, Yoshimi R, Taguri M, Kimura K, Hibi K, Wakui H, Azushima K, Tamura K, On Behalf Of Oracle Arthritis Investigators.
Rationale and Design of the Orencia Atherosclerosis and Rheumatoid Arthritis Study (ORACLE Arthritis Study): Implications of Biologics against Rheumatoid Arthritis and the Vascular Complications, Subclinical Atherosclerosis.
Methods Protoc. 2021 Nov 15;4(4):83. Open access
doi: 10.3390/mps4040083. PMID: 34842780
 
Nishiyama K, Maekawa M, Nakagita T, Nakayama J, Kiyoi T, Chosei M, Murakami A, Kamei Y, Takeda H, Takada Y, Higashiyama S.
CNKSR1 serves as a scaffold to activate an EGFR phosphatase via exclusive interaction with RhoB-GTP.
Life Sci Alliance. 2021 Jun 29;4(9):e202101095. Open access
doi: 10.26508/lsa.202101095. PMID: 34187934; PMCID: PMC8321701.
 
Tachibana K, Hashimoto Y, Shirakura K, Okada Y, Hirayama R, Iwashita Y, Nishino I, Ago Y, Takeda H, Kuniyasu H, Kondoh M.
Safety and efficacy of an anti-claudin-5 monoclonal antibody to increase blood-brain barrier permeability for drug delivery to the brain in a non-human primate.
J Control Release. 2021 Aug 10;336:105-111.
doi: 10.1016/j.jconrel.2021.06.009. Epub 2021 Jun 9. PMID: 34118338.
 
Zhou W, Kaneko N, Nakagita T, Takeda H*, Masumoto J*.
A comprehensive interaction study provides a potential domain interaction network of human death domain superfamily proteins.
Cell Death Differ. 2021 Nov;28(11):2991-3008. Open Access High Impact Factor Journal
doi: 10.1038/s41418-021-00796-x. Epub 2021 May 15. PMID: 33993194
 
Yamanaka S, Murai H, Saito D, Abe G, Tokunaga E, Iwasaki T, Takahashi H, Takeda H, Suzuki T, Shibata N, Tamura K, Sawasaki T.
Thalidomide and its metabolite 5-hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF.
EMBO J. 2021 Feb 15;40(4):e105375. High Impact Factor Journal
doi: 10.15252/embj.2020105375. Epub 2021 Jan 20. PMID: 33470442
 
Yuguchi T, Kanoi BN, Nagaoka H, Miura T, Ito D, Takeda H, Tsuboi T, Takashima E, Otsuki H.
Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein.
Front Cell Infect Microbiol. 2021 Apr 14;11:656620.
doi: 10.3389/fcimb.2021.656620. PMID: 33937099

Wei Zhou, Hiroyuki Takeda.
Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins.
Journal of visualized experiments : JoVE (2020)
DOI: 10.3791/61871
 
Kaneko, N., Kurata, M., Yamamoto, T., Shigemura, T., Agematsu, K., Yamazaki, T., Takeda, H., Sawasaki, T., Koga, T., Kawakami, A., Yachie, A., Migita, K., Yoshiura, K. I., Urano, T., & Masumoto, J.
KN3014, a piperidine-containing small compound, inhibits auto-secretion of IL-1β from PBMCs in a patient with Muckle-Wells syndrome. 
Scientific reports, 10(1), 13562. (2020) Open Access
https://doi.org/10.1038/s41598-020-70513-0
 
Miyoshi S, Tokunaga S, Ozawa T, Takeda H, Aono M, Miyoshi T, Kishi H, Muraguchi A, Shimizu SI, Nozawa A, Sawasaki T. Production of a rabbit monoclonal antibody for highly sensitive detection of citrus mosaic virus and related viruses. PLoS One. 2020 Apr 15;15(4):e0229196.  Open Access
doi: 10.1371/journal.pone.0229196. 

Chigira T, Nagatoishi S, Takeda H, Yoshimaru T, Katagiri T, Tsumoto K.
Biophysical characterization of the breast cancer-related BIG3-PHB2 interaction: Effect of non-conserved loop region of BIG3 on the structure and the interaction. Biochem Biophys Res Commun. 518:183-189. (2020)
doi: 10.1016/j.bbrc.2019.08.028.

Yosuke Hashimoto, Wei Zhou, Kohtaroh Hamauchi, Keisuke Shirakura, Takefumi Doi, Kiyohito Yagi, Tatsuya Sawasaki, Yoshiaki Okada, Masuo Kondoh*, Hiroyuki Takeda*. （*co-correspondent author)
Engineered membrane protein antigens successfully induce antibodies against extracellular regions of claudin-5. Sci Rep. 8:8383.  (2020)
doi:10.1038/s41598-018-26560-9.
 
Suzuki Y, Ogasawara T, Tanaka Y, Takeda H, Sawasaki T, Mogi M, Liu S, Maeyama K. Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The Pharmacological Analysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes. Front Pharmacol. 2018 Feb 6;9:38. doi: 10.3389/fphar.2018.00038.
 
Uematsu A, Kido K, Manabe E, Takeda H, Takahashi H, Hayashi M, Imai Y, Sawasaki T. DANFIN functions as an inhibitor of transcription factor NF-κB and potentiates the antitumor effect of bortezomib in multiple myeloma. Biochem Biophys Res Commun. 2018 Jan 15;495(3):2289-2295. doi: 10.1016/j.bbrc.2017.12.142. Epub 2017 Dec 25. PubMed PMID: 29284118.

Liu S, Hasegawa H, Takemasa E, Suzuki Y, Oka K, Kiyoi T, Takeda H, Ogasawara T, Sawasaki T, Yasukawa M, Maeyama K. Efficiency and Safety of CRAC Inhibitors in Human Rheumatoid Arthritis Xenograft Models. J Immunol. 2017 Jul 17. pii: ji1700192. doi: 10.4049/jimmunol.1700192. 
 
Kaneko N, Ito Y, Iwasaki T, Takeda H, Sawasaki T, Migita K, Agematsu K, Koga T, Kawakami A, Yachie A, Yoshiura K, Morikawa S, Kurata M, Masumoto J. (2017)
Poly (I:C) and hyaluronic acid directly interact with NLRP3, resulting in the assembly of NLRP3 and ASC in a cell-free system. European Journal of Inflammation. DOI: https://doi.org/10.1177/1721727X17711047. Open Access
 
Takeda H, Zhou W, Kido K, Suno R, Iwasaki T, Kobayashi T, Sawasaki T. (2017) CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag. PLoS One. 12:e0178246. doi: 10.1371/journal.pone.0178246. Open Access
 
Kaneko N, Iwasaki T, Ito Y, Takeda H, Sawasaki T, Morikawa S, Nakano N, Kurata M,  Masumoto J.  Applications of reconstituted inflammasomes in a cell-free system to drug discovery and elucidation of the pathogenesis of autoinflammatory diseases. Inflammation and Regeneration201737:9  DOI: 10.1186/s41232-017-0040-y  Open Access
 
Yamamoto T, Taira Nihira N, Yogosawa S, Aoki K, Takeda H, Sawasaki T, Yoshida K. Interaction between RNF8 and DYRK2 is required for the recruitment of DNA repair molecules to DNA double-strand breaks. FEBS Lett. 2017 Mar;591(6):842-853. doi: 10.1002/1873-3468.12596.

Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, Tokunaga F. (2016) Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun.  7:12547. doi: 10.1038/ncomms12547. 
 
Iwasaki T, Kaneko N, Ito Y, Takeda H, Sawasaki T, Heike T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J. (2016) Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset Sarcoidosis. ScientificWorldJournal. 2597376. doi: 10.1155/2016/2597376.
 
Yano T, Takeda H, Uematsu A, Yamanaka S, Nomura S, Nemoto K, Iwasaki T, Takahashi H, Sawasaki T. (2016) AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis. PLoS One. 11:e0156716. doi: 10.1371/journal.pone.0156716. Open Access
 
Takahashi H, Uematsu A, Yamanaka S, Imamura M, Nakajima T, Doi K, Yasuoka S, Takahashi C, Takeda H, Sawasaki T. (2016) Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins. PLoS One. 11:e0156718. doi: 10.1371/journal.pone.0156718. Open Access

Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima  M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T. (2015) Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay. Sci Rep. 5:11333. Open Access
 
 Ramadan A, Nemoto K, Seki M, Shinozaki K, Takeda H, Takahashi H, Sawasaki T. (2015) Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes. BMC Plant Biol. 15:275. 
 
 Kaneko　N, Ito Y, Iwasaki T, Takeda H, Sawasaki T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J (2015) Reconstituted AIM2 inflammasome in cell-free system. J Immunol Methods 426:76-81.
 
 Ohnishi T, Yanazawa M, Sasahara T, Kitamura Y, Hiroaki H, Fukazawa Y, Kii I, Nishiyama T, Kakita A, Takeda H, Takeuchi A, Arai Y, Ito A, Komura H, Hirao H, Satomura K, Inoue M, Muramatsu SI, Matsui K, Tada M, Sato M, Saijo E, Shigemitsu  Y, Sakai S, Umetsu Y, Goda N, Takino N, Takahashi H, Hagiwara M, Sawasaki T, Iwasaki G, Nakamura Y, Nabeshima YI, Teplow DB, Hoshi M. (2015) Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. Proc Natl Acad Sci U S A. 112:E4465-74.
 
 Nakajia M, Nagase S, Iida M, Takeda S, Yamashita M, Watari A, Shirasago Y, Fukasawa M, Takeda H, Sawasaki T, Yagi K, Kondoh M. (2015) Claudin-1 binder enhances epidermal permeability in a human keratinocyte model. J Pharmacol Exp Ther. 354:440-447.
 
 Onishi S, Adnan E, Ishizaki J, Miyazaki T, Tanaka Y, Matsumoto T, Suemori K, Shudou M, Okura T, Takeda H, Sawasaki T, Yasukawa M, Hasegawa H. (2015) Novel Autoantigens Associated with Lupus Nephritis. PLoS One. 10:e0126564. 2014

Arimitsu E, Ogasawara T, Takeda H, Sawasaki T, Ikeda Y, Hiasa Y, Maeyama K. (2014) The ligand binding ability of dopamine D1 receptors synthesized using a wheat germ cell-free protein synthesis system with liposomes. Eur J Pharmacol. 745:117-122.
 
 Takemori N, Takemori A, Matsuoka K, Morishita R, Matsushita N, Aoshima M, Takeda H, Sawasaki T, Endo Y, Higashiyama S. (2014) High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system. Mol Biosyst. 11:361-365.
 
 Mizutani Y, Tsuge S, Takeda H, Hasegawa Y, Shiogama K, Onouchi T, Inada K, Sawasaki T, Tsutsumi Y. (2014) In situ visualization of plasma cells producing antibodies reactive to Porphyromonas gingivalis in periodontitis: the application of the enzyme-labeled antigen method. Mol Oral Microbiol. 29:156-73.
 
 Nagayoshi Y, Nakamura M, Matsuoka K, Ohtsuka T, Mori Y, Kono H, Aso T, Ideno N, Takahata S, Ryo A, Takeda H, Ito T, Oda Y, Endo Y, Sawasaki T, Tanaka M. (2014) Profiling of autoantibodies in sera of pancreatic cancer patients. Ann Surg Oncol. Suppl 3:S459-465.
 
 Takiuchi T, Nakagawa T, Tamiya H, Fujita H, Sasaki Y, Saeki Y, Takeda H, Sawasaki T, Buchberger A, Kimura T, Iwai K. (2014) Suppression of LUBAC-mediated linear ubiquitination by a specific interaction between LUBAC and the deubiquitinases CYLD and OTULIN. Genes Cells. 19:254-272.

Ishigami T, Abe K, Aoki I, Minegishi S, Ryo A, Matsunaga S, Matsuoka K, Takeda H, Sawasaki T, Umemura S, Endo Y. (2013) Anti-interleukin-5 and multiple autoantibodies are associated with human atherosclerotic diseases and serum interleukin-5 levels. FASEB J. 27:3437-3445.
 
 Mizutani Y, Matsuoka K, Takeda H, Shiogama K, Inada K, Hayakawa K, Yamada H, Miyazaki T, Sawasaki T, Endo Y, Tsutsumi Y. (2013) Novel approach to identifying autoantibodies in rheumatoid synovitis with a biotinylated human autoantigen library and the enzyme-labeled antigen method. J Immunol Methods. 387:57-70. 

Takahashi H, Ozawa A, Nemoto K, Nozawa A, Seki M, Shinozaki K, Takeda H, Endo Y, Sawasaki T. (2012) Genome-wide biochemical analysis of Arabidopsis protein phosphatase using a wheat cell-free system. FEBS Lett. 586:3134-3141.  

Takeda H, Yoneyama F, Kawai S, Hashimoto W, and Murata K. (2011) Bioethanol production from marine biomass alginate by metabolically engineered bacteria. Ener Environ Sci 4 2575 – 2581.

Takeda H, Kawamura Y, Miura A, Mori M, Wakamatsu A, Yamamoto J, Isogai T, Matsumoto M, Nakayama K, Natsume T, Goshima N and Nomura N. (2010) Comparative Analysis of Human Src-Family Kinase substrate specificity in vitro. J Proteome Res 9 5982 – 5993.
 
 Takeda H, Sugahara T, Kotake T, Nakagawa N and Sakurai N. (2010) Sugar treatment inhibits IAA-induced expression of endo-1,3:1,4-β-glucanase EI transcripts in barley coleoptile segments. Physiol Plantarum 139, 413-420.
 
 Goshima N, Kawamura Y, Fukumoto A, Miura A, Honma R, Satoh R, Wakamatsu A, Yamamoto J,Kimura K, Nishikawa T, Andoh T, Iida Y, Ishikawa K, Ito E, Kagawa N, Kaminaga C, Kanehori K,Kawakami B, Kenmochi K, Kimura R, Kobayashi M, Kuroita T, Kuwayama H, Maruyama Y, Matsuo K,Minami K, Mitsubori M, Mori M, Morishita R, Murase A, Nishikawa A, Nishikawa S, Okamoto T,Sakagami N, Sakamoto Y, Sasaki Y, Seki T, Sono S, Sugiyama A, Sumiya T, Takayama T, Takayama Y, Takeda H, Togashi T, Yahata K, Yamada H, Yanagisawa Y, Endo Y, Imamoto F, Kisu Y, Tanaka S, Isogai T, Imai J, Watanabe S, Nomura N. (2008) Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility. Nat Methods 5, 1011-1017.
 
 Takeda H, Ueno A, Miura A, Goshima N and Nomura N. (2006) High-throughput kinase assay based on surface plasmon resonance suitable for native protein substrates. Anal Biochem 357, 262-71.
 
 Takeda H, Yoshikawa T, Liu XZ, Nakagawa N, Li YQ and Sakurai N. (2004) Molecular cloning of two exo-β-glucanases and their in vivo substrates in the cell walls of lily pollen tubes. Plant Cell Physiol 45, 436-444.
 
 Zhou W, Takeda H, Liu XZ, Nakagawa N, Sakurai N, Huang J and Li YQ. (2004) A novel endo-1,4-β-glucanase gene (LlpCel1) is exclusively expressed in pollen and pollen tubes of Lilium longiflorum. Acta Bot Sin 46, 142-147.
 
 Takeda H, Kotake T, Nakagawa N, Sakurai N and Nevins DJ (2003) Expression and function of cell wall-bound cationic peroxidase in asparagus somatic embryogenesis. Plant Physioly 131, 1765-1774.