【2024】

1. "Cell-Free Biosensors Based on Modular Eukaryotic Riboswitches That Function in One Pot at Ambient Temperature"
   Atsushi Ogawa*, Masahiro Fujikawa, Kazuki Onishi, Hajime Takahashi
   ACS Synth. Biol. 2024, 13, 2238-2245. [link]

【2023】

1. "Effect of DNA density immobilized on gold nanoparticles on nucleic acid detection"
   Gen Hirao, Nanami Fukuzumi, Atsushi Ogawa, Tsuyoshi Asahi, Mizuo Maeda, Tamotsu Zako*
   RSC Adv. 2023, 13, 30690-30695. [link]

2. "Rational design of eukaryotic riboswitches that upregulate IRES-mediated translation initiation with high switching efficiency through a kinetic trapping mechanism in vitro"
   Hajime Takahashi, Masahiro Fujikawa, Atsushi Ogawa*
   RNA 2023, 29, 1950-1959. [link]

3. "Facile Expansion of the Variety of Orthogonal Ligand/Aptamer Pairs for Artificial Riboswitches"
   Atsushi Ogawa*, Honami Inoue, Yu Itoh, Hajime Takahashi
   ACS Synth. Biol. 2023, 12, 35-42. [link]

【2022】

1. "Eukaryotic artificial ON-riboswitches that respond efficiently to mid-sized short peptides"
   Hajime Takahashi, Ryo Okubo, Atsushi Ogawa*
   Bioorg. Med. Chem. Lett. 2022, 71, 128839. [link]

【2021】

1. "Coupled in vitro transcription/translation based on wheat germ extract for efficient expression from PCR-generated templates in short-time batch reactions"
   Hajime Takahashi and Atsushi Ogawa*
   Bioorg. Med. Chem. Lett. 2021, 52, 128412. [link]

2. "A Detailed Protocol for Preparing Millimeter-Sized Supergiant Liposomes That Permit Efficient Eukaryotic Cell-Free Translation in the Interior" [Protocol]
   Hajime Takahashi and Atsushi Ogawa*
   Bio-protocol 2021, 11, e4054. [link]
   #selected as the outside front cover

3. "Molecular detection using aptamer-modified gold nanoparticles with an immobilized DNA brush for the prevention of non-specific aggregation"
   Yuki Yano-Ozawa, Nadine Lobsiger, Yu Muto, Takahiro Mori, Ken Yoshimura, Yuki Yano, Wendelin Jan Stark, Mizuo Maeda, Tsuyoshi Asahi, Atsushi Ogawa, Tamotsu Zako*
   RSC Adv. 2021, 11, 11984-11991. [link]

4. "Suppressor tRNA-based Biosensors for Detecting Analytes" [Review]
   Atsushi Ogawa*
   Anal. Sci. 2021, 37, 407-414. [link]

【2020】

1. "In Vitro Selection of RNA Aptamers Binding to Nanosized DNA for Constructing Artificial Riboswitches"
   Atsushi Ogawa* and Yu Itoh
   ACS Synth. Biol. 2020, 9, 2648-2655. [link]

2. "Development of human CBF1-targeting single-stranded DNA aptamers with anti-angiogenic activity in vitro"
   Mari Tezuka-Kagajo(#), Masashi Maekawa(#),* Atsushi Ogawa(#),* Yoshiko Hatta, Eiichi Ishii, Mariko Eguchi, Shigeki Higashiyama*
   Nucleic Acid Ther. 2020, 30, 365-378. [link]
   (#)These authors equally contributed to this work.

3. "Preparation of a Millimeter-Sized Supergiant Liposome That Allows for Efficient, Eukaryotic Cell-Free Translation in the Interior by Spontaneous Emulsion Transfer"
   Hajime Takahashi and Atsushi Ogawa*
   ACS Synth. Biol. 2020, 9, 1608-1614. [link]

【2019】

1. "Mutation of the start codon to enhance Cripavirus internal ribosome entry site-mediated translation in a wheat germ extract"
   Atsushi Ogawa* and Masashi Takamatsu
   Bioorg. Med. Chem. Lett. 2019, 29, 126729. [link]

2. "In vitro selection of a 3’ terminal short protector that stabilizes transcripts to improve the translation efficiency in a wheat germ extract"
   Atsushi Ogawa,* Akane Kutsuna, Masashi Takamatsu, Tatsuya Okuzono
   Bioorg. Med. Chem. Lett. 2019, 29, 2141-2144. [link]

3. "Detection of Gold Nanoparticles Aggregation using Light Scattering for Molecular Sensing"
   Yuki Yano, Masamichi Nisougi, Yuki Yano-Ozawa, Tsuyoshi Ohguni, Atsushi Ogawa, Mizuo Maeda, Tsuyoshi Asahi, Tamotsu Zako*
   Anal. Sci. 2019, 35, 685-690. [link]
   #selected as a hot article

4. "Modification of carbon metabolism in Synechococcus elongatus PCC 7942 by cyanophage-derived sigma factors for bioproduction improvement"
   Naruhiko Sawa, Tsuneyuki Tatsuke, Atsushi Ogawa, Yasutaka Hirokawa, Takashi Osanai, Taizo Hanai*
   J. Biosci. Bioeng. 2019, 127, 256-264. [link]

【2018】

1. "Canonical translation-modulating OFF-riboswitches with a single aptamer binding to a small molecule that function in a higher eukaryotic cell-free expression system"
   Atsushi Ogawa,* Yuta Murashige, Hajime Takahashi
   Bioorg. Med. Chem. Lett. 2018, 28, 2353-2357. [link]

2. "Rational Design of Artificial Riboswitches" [Review]
   Atsushi Ogawa*
   Applied RNA Bioscience 2018, 79-93. [link]

【2017】

1. "Artificial OFF-Riboswitches That Downregulate Internal Ribosome Entry without Hybridization Switches in a Eukaryotic Cell-Free Translation System"
   Atsushi Ogawa,* Hiroki Masuoka, Tsubasa Ota
   ACS Synth. Biol. 2017, 6, 1656-1662. [link]

2. "Ligand-responsive upregulation of 3' CITE-mediated translation in a wheat germ cell-free expression system"
   Atsushi Ogawa,* Yuta Murashige, Junichiro Tabuchi, Taiki Omatsu
   Mol. BioSyst. 2017, 13, 314-319. [link]

【2016】

1. "Biofunction-assisted DNA detection through RNase H-enhanced 3' processing of a premature tRNA probe in a wheat germ extract"
   Atsushi Ogawa,* Junichiro Tabuchi, Yasunori Doi, Masashi Takamatsu
   Bioorg. Med. Chem. Lett. 2016, 26, 3658-3661. [link]

2. "Rational optimization of amber suppressor tRNAs toward efficient incorporation of a non-natural amino acid into protein in a eukaryotic wheat germ extract"
   Atsushi Ogawa,* Yuki Namba, Mai Gakumasawa
   Org. Biomol. Chem. 2016, 14, 2671-2678. [link]

【2015】

1. "Biofunction-assisted aptasensors based on ligand-dependent 3’ processing of a suppressor tRNA in a wheat germ extract"
   Atsushi Ogawa* and Junichiro Tabuchi
   Org. Biomol. Chem. 2015, 13, 6681-6685. [link]
   #selected as a hot article
   #selected as the inside front cover
   (Correction: Org. Biomol. Chem. 2015, 13, 8365. [link])

2. "Engineering of Ribosomal Shunt-Modulating Eukaryotic ON Riboswitches by Using a Cell-Free Translation System" [Protocol]
   Atsushi Ogawa*
   Methods Enzymol. 2015, 550, 109-128. [link]

3. "Investigation of end processing and degradation of premature tRNAs and their application to stabilization of in vitro transcripts in wheat germ extract"
   Atsushi Ogawa* and Yasunori Doi
   Org. Biomol. Chem. 2015, 13, 1008-1012. [link]

【2014】

1. "Thermodynamics-based Rational Design of DNA Block Copolymers for Quantitative Detection of Single-Nucleotide Polymorphisms by Affinity Capillary Electrophoresis"
   Ayumi Kimura, Naoki Kanayama, Atsushi Ogawa, Hideaki Shibata, Hideo Nakashita, Tohru Takarada,* Mizuo Maeda
   Anal. Chem. 2014, 86, 11425-11433. [link]

2. "Identification of short untranslated regions that sufficiently enhance translation in high-quality wheat germ extract"
   Atsushi Ogawa,* Junichiro Tabuchi, Yasunori Doi
   Bioorg. Med. Chem. Lett. 2014, 24, 3724-3727. [link]

3. "Rational Design of Artificial ON-Riboswitches" [Protocol]
   Atsushi Ogawa*
   Methods Mol. Biol. 2014, 1111, 165-181. [link]

【2013】

1. "Theophylline-dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC 7942"
   Yoichi Nakahira, Atsushi Ogawa, Hiroyuki Asano, Tokitaka Oyama, Yuzuru Tozawa*
   Plant Cell Physiol. 2013, 54, 1724-1735. [link]

2. "Ligand-Dependent Upregulation of Ribosomal Shunting"
   Atsushi Ogawa*
   ChemBioChem 2013, 14, 1539-1543. [link]
   #selected as a very important paper (VIP)
   #selected as the outside front cover

3. "Dumbbell-Shaped DNA Analytes Amplified by Polymerase Chain Reaction for Robust Single-Nucleotide Polymorphism Genotyping by Affinity Capillary Electrophoresis"
   Hideaki Shibata, Atsushi Ogawa, Naoki Kanayama, Tohru Takarada,* Mizuo Maeda
   Anal. Chem. 2013, 85, 5347-5352. [link]

4. "Multiple-input and visible-output logic gates using signal-converting DNA machines and gold nanoparticle aggregation"
   Atsushi Ogawa* and Yukiko Susaki
   Org. Biomol. Chem. 2013, 11, 3272-3276. [link]
   #selected as the outside front cover

【2012】

1. "A Concept for Selection of Codon-Suppressor tRNAs Based on Read-Through Ribosome Display in an In Vitro Compartmentalized Cell-Free Translation System"
   Atsushi Ogawa,* Masayoshi Hayami, Shinsuke Sando,* Yasuhiro Aoyama
   J. Nucleic Acids 2012, Article ID 538129, 1-7. [link]

2. "Rational construction of eukaryotic OFF-riboswitches that downregulate internal ribosome entry site-mediated translation in response to their ligands"
   Atsushi Ogawa*
   Bioorg. Med. Chem. Lett. 2012, 22, 1639-1642. [link]

【2011】

1. "Improvement of in vitro-transcribed amber suppressor tRNAs toward higher suppression efficiency in wheat germ extract"
   Atsushi Ogawa,* Yasunori Doi, Nobuto Matsushita
   Org. Biomol. Chem. 2011, 9, 8495-8503. [link]

2. "Multiple-Catalytic Sensing of Nucleic Acid Sequences by Utilising a DNA-RNA-DNA Chimeric Antisense Probe and RNase H with a Eukaryotic Cell-Free Translation System"
   Atsushi Ogawa*
   ChemBioChem 2011, 12, 881-885. [link]

3. "Rational Design of Artificial Riboswitches Based on Ligand-Dependent Modulation of Internal Ribosome Entry in Wheat Germ Extract and Their Applications as Label-Free Biosensors"
   Atsushi Ogawa*
   RNA 2011, 17, 478-488. [link]

4. "RNA aptazyme-tethered large gold nanoparticles for on-the-spot sensing of the aptazyme ligand"
   Atsushi Ogawa*
   Bioorg. Med. Chem. Lett. 2011, 21, 155-159. [link]

【2010】

1. "Isothermal Sensitive Detection of MicroRNA Using an Autonomous DNA Machine Recycling Output as Input"
   Atsushi Ogawa*
   Bioorg. Med. Chem. Lett. 2010, 20, 6056-6060. [link]

【2009】

1. "Biofunction-Assisted Sensors Based on a New Method for Converting Aptazyme Activity into Reporter Protein Expression with High Efficiency in Wheat Germ Extract"
   Atsushi Ogawa*
   ChemBioChem 2009, 10, 2465-2468. [link]

2. "Easy design of logic gates based on aptazymes and noncrosslinking gold nanoparticle aggregation"
   Atsushi Ogawa* and Mizuo Maeda*
   Chem. Commun. 2009, 4666-4668. [link]
   #highlighted in the magazine "Highlights in Chemical Science"

3. "Detector-free and Multiple Sensing of Various Molecules Using Gold Nanoparticles and Aptazymes"
   Atsushi Ogawa* and Mizuo Maeda*
   Chem. Lett. 2009, 38, 848-849. [link]

【2008】

1. "A Novel Label-Free Biosensor Using an Aptazyme-Suppressor-tRNA Conjugate and an Amber-Mutated Reporter Gene"
   Atsushi Ogawa* and Mizuo Maeda
   ChemBioChem 2008, 9, 2204-2208. [link]

2. "Simple and Rapid Colorimetric Detection of Cofactors of Aptazymes Using Noncrosslinking Gold Nanoparticle Aggregation"
   Atsushi Ogawa* and Mizuo Maeda*
   Bioorg. Med. Chem. Lett. 2008, 18, 6517-6520. [link]

3. "An Artificial Aptazyme-Based Riboswitch and its Cascading System in E. Coli"
   Atsushi Ogawa* and Mizuo Maeda
   ChemBioChem 2008, 9, 206-209. [link]
   # Most Cited Articles among those published in ChemBioChem in 2007 or 2008 (Top 25)

【2007】

1. "Aptazyme-Based Riboswitches as Label-Free and Detector-Free Sensors for Cofactors"
   Atsushi Ogawa* and Mizuo Maeda
   Bioorg. Med. Chem. Lett. 2007, 17, 3156-3160. [link]

1. "30歳単身から始めた地方での研究室運営十数年"
   小川 敦司
   日本化学会 生体機能関連化学部会NEWS LETTER 2020, 35, 34-35. [link]

2. "Methods in Molecular Biology: Artificial Riboswitches"
   Atsushi Ogawa (Editor)
   Springer 2014. [link]

3. Book Review on "Methods in Molecular Biology: Riboswitches"
   Atsushi Ogawa
   ChemBioChem (Wiley) 2009, 10, 2802-2803. [link]

4. "金テクノロジー　−その基礎と応用−：第23章「金ナノ粒子を用いるバイオセンシング」"
   小川 敦司、前田 瑞夫
   シーエムシー出版 2009, 294-308. [link]

5. "機能性核酸を用いたターゲット分子簡易検出システムの開発"
   小川 敦司、前田 瑞夫
   日本化学会 生体機能関連化学部会NEWS LETTER 2008, 23, 6-9. [link]