{"id":253,"date":"2026-03-21T10:38:55","date_gmt":"2026-03-21T01:38:55","guid":{"rendered":"https:\/\/www.pros.ehime-u.ac.jp\/Interactome\/?page_id=253"},"modified":"2026-06-09T18:22:26","modified_gmt":"2026-06-09T09:22:26","slug":"101-2","status":"publish","type":"page","link":"https:\/\/www.pros.ehime-u.ac.jp\/Interactome\/yamanakalab\/101-2\/","title":{"rendered":"Publish"},"content":{"rendered":"\n
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\u7814\u7a76\u696d\u7e3e \/ \u8457\u66f8\u30fb\u89e3\u8aac<\/p>\n<\/h2><\/div>\n\n\n\n

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1) \u7814\u7a76\u696d\u7e3e<\/h4>\n
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  1. An interactome-based framework for DDB1- and CUL4-associated factor prioritization in targeted protein degradation. \nSatoshi Yamanaka, Koya Nagaoka, Yuki Shoya, Kohei Nishino, Yumi Mikura, Kenshi Tanaka, Keisuke Konishi, Yoshinori Hasegawa, Atsushi Hijikata, Hidetaka Kosako \u2026\nMolecular cell 86(7) 1397-1416 2026\u5e744\u67082\u65e5 <\/li>\n \n\n
  2. Alkyne Two-Phase Strategy: Rapid Generation of TK-285-Derived PROTACs as BRD4 Degraders. \nHiroyuki Yamakoshi, Ryo Watanabe, Ryosuke Segawa, Ryosuke Ishihara, Ryo Tachibana, Genki Kudo, Shota Nagasawa, Satoshi Yamanaka, Ayano Ito, Hiroyuki Takeda \u2026\nJournal of medicinal chemistry. 2026\u5e743\u670819\u65e5\n<\/li>\n \n
  3. In-cell proximity target validation methods for heterobifunctional molecules with CRBN- or VHL-binder using AirID. Kohdai Yamada, Satoshi Yamanaka, Hiroyuki Yamakoshi, Aki Kohyama, Yoshiharu Iwabuchi, Hidetaka Kosako, Tatsuya Sawasaki ,Communications Biology 8(1) 2025\u5e748\u670830\u65e5 <\/li>\n\t\t  \n
  4. Motoi Yamashita, Chihiro Ogawa, Baihao Zhang, Tetsuro Kobayashi, Aneela Nomura, Clive Barker, Chengcheng Zou, Satoshi Yamanaka, Ken-Ichiro Hayashi, Yoichi Shinkai, Kazuyo Moro, Sidonia Fargarasan, Koshi Imami, Jun Seita, Fumiyuki Shirai, Tatsuya Sawasaki, Masato T Kanemaki, Ichiro Taniuchi. Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems, Nature Communications, 15, 10129, (2024)<\/li>\n\t\t  \n
  5. Satoshi Yamanaka. Targeted protein degradation using thalidomide and its derivatives, Translational and Regulatory Sciences, 6, 51-59, (2024)<\/li>\n\t\t  \n
  6. Yuki Utsugi, Ken Nishimura, Satoshi Yamanaka, Kohei Nishino, Hidetaka Kosako, Tatsuya Sawasaki, Hideyuki Shigemori, Thomas J Wandless, Yusaku Miyamae. Ubiquitin-Derived Fragment as a Peptide Linker for the Efficient Cleavage of a Target Protein from a Degron, ACS Chemical Biology, 19, 497-505, (2024)\n\t\t <\/li> \n
  7. Satoshi Yamanaka, Hirotake Furihata, Yuta Yanagihara, Akihito Taya, Takato Nagasaka, Mai Usui, Koya Nagaoka, Yuki Shoya, Kohei Nishino, Shuhei Yoshida, Hidetaka Kosako, Masaru Tanokura, Takuya Miyakawa, Yuuki Imai, Norio Shibata, Tatsuya Sawasaki. Lenalidomide derivatives and proteolysis-targeting chimaeras for controlling neosubstrate degradation, Nature Communications, 14, 4683, (2023)<\/li> \n\n
  8. A strategy for orthogonal deubiquitination using a bump-and-hole approach \nTakumi Suzuki, Yuki Utsugi, Satoshi Yamanaka, Hirotaka Takahashi, Yusuke Sato, Tatsuya Sawasaki, Yusaku Miyamae\nRSC Chemical Biology 4(11) 879-883 (2023) <\/li> \n\t\t \n
  9. Satoshi Yamanaka, Yuto Horiuchi, Saya Matsuoka, Kohki Kido, Kohei Nishino, Mayaka Maeno, Norio Shibata, Hidetaka Kosako, Tatsuya Sawasaki. A proximity biotinylation-based approach to identify protein-E3 ligase interactions induced by PROTACs and molecular glues, Nature Communications, 13, 183, (2022)\n\t\t <\/li> \n
  10. Shusei Sugiyama#, Kohdai Yamada#, Miwako Denda, Satoshi Yamanaka, Satoshi Ozawa, Ryo Morishita, Tatsuya Sawasaki (#equally contributed). CF-PPiD technology based on cell-free protein array and proximity biotinylation enzyme for in vitro direct interactome analysis, Scientific Reports, 12, 10592, (2022)\n\t\t <\/li> \n
  11. Satoshi Yamanaka, Hidetaka Murai, Daisuke Saito, Gembu Abe, Etsuko Tokunaga, Takahiro Iwasaki, Hirotaka Takahashi, Hiroyuki Takeda, Takayuki Suzuki, Norio Shibata, Koji Tamura, Tatsuya Sawasaki. Thalidomide and its metabolite 5-hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF, EMBO Journal, 40, e105375, (2021)\n\t\t <\/li> \n
  12. Satoshi Yamanaka, Yuki Shoya, Saya Matsuoka, Hisayo Nishida-Fukuda, Norio Shibata, Tatsuya Sawasaki. An IMiD-induced SALL4 degron system for selective degradation of target proteins, Communications Biology, 3, 515, (2020)\n\t\t <\/li> \n
  13. Hirotake Furihata#, Satoshi Yamanaka#, Toshiaki Honda, Yumiko Miyauchi, Atsuko Asano, Norio Shibata, Masaru Tanokura, Tatsuya Sawasaki, Takuya Miyakawa (#equally contributed). Structural bases of IMiD selectivity that emerges by 5-hydroxythalidomide, Nature Communications, 11, 4578, (2020)\n\t\t <\/li> \n
  14. Satoshi Yamanaka, Yusuke Sato, Daisuke Oikawa, Eiji Goto, Shuya Fukai, Fuminori Tokunaga, Hirotaka Takahashi, Tatsuya Sawasaki. Subiquinocin, a small molecule inhibitor of CYLD and USP family deubiquitinating enzymes, promotes NF-\u03baB signaling, Biochemical and Biophysical Research Communications, 524, 1\u20137, (2020).\n\t\t <\/li> \n
  15. Hirotaka Takahashi, Satoshi Yamanaka, Shohei Kuwada, Kana Higaki, Kohki kido, Yusuke Sato, Shuya Fukai, Fuminori Tokunaga, Tatsuya Sawasaki. Subiquinocin, a small molecule inhibitor of CYLD and USP family deubiquitinating enzymes, promotes NF-\u03baB signaling, Biomedicines, 8, 152, (2020).\n\t\t <\/li> \n
  16. Koki Kido, Satoshi Yamanaka, Shogo Nakano, Kou Motani, Souta Shinohara, Akira Nozawa, Hidetaka Kosako, Sohei Ito, and Tatsuya Sawasaki. AirID, a novel proximity biotinylation enzyme, for analysis of protein-protein interactions, eLife, e54983, (2020)\n\t\t <\/li> \n
  17. Keiichirou Nemoto, Akira Nozawa, Satoshi Yamanaka, Shunsuke Nomura, Kohki Kido, and Tatsuya Sawasaki. Autophosphorylation Assays Using Plant Receptor Kinases Synthesized in Cell-Free Systems, Methods in Molecular Biology, 1621, 113-120, (2017)\n\t\t <\/li> \n
  18. Akira Nozawa, Keiichirou Nemoto, Shunsuke Nomura, Satoshi Yamanaka, Kohki Kido, and Tatsuya Sawasaki. Cell-Free Synthesis of Plant Receptor Kinases. Methods in Molecular Biology, 1621, 37-46, (2017).\n\t\t <\/li> \n
  19. Tomoya Yano, Hiroyuki Takeda, Atsushi Uematsu, Satoshi Yamanaka, Shunsuke Nomura, Keiichirou Nemoto, Takahiro Iwasaki, Hirotaka Takahashi, Tatsuya Sawasaki. AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis. PLOS ONE, 11, e0156716, (2016)\n\t\t <\/li> \n
  20. Hirotaka Takahashi, Atsushi Uematsu, Satoshi Yamanaka, Mei Imamura, Tatsuro Nakajima, Kousuke Doi, Saki Yasuoka, Chikako Takahashi, Hiroyuki Takeda, Tatsuya Sawasaki. Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins. PLOS ONE, 11, e0156718, (2016)\n\t\t <\/li> \n
  21. AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis. Tomoya Yano, Hiroyuki Takeda, Atsushi Uematsu, Satoshi Yamanaka, Shunsuke Nomura, Keiichirou Nemoto, Takahiro Iwasaki, Hirotaka Takahashi, Tatsuya Sawasaki, PloS one 11(6) e0156716 2016\u5e74 \n\t\t\t<\/li> \n\n \n \n<\/div>\n\n\n\n
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    2) \u8457\u66f8\u3001\u89e3\u8aac<\/h4>\n \n
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    1. \u30ea\u30a2\u30eb\u306a\u76f8\u4e92\u4f5c\u7528\u3092\u6349\u3048\u308b\u8fd1\u4f4d\u4f9d\u5b58\u6027\u6a19\u8b58\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb. \u5c71\u4e2d \u8061\u58eb. \u7f8a\u571f\u793e: \u5b9f\u9a13\u533b\u5b66\u5225\u518a \u6700\u5f37\u306e\u30b9\u30c6\u30c3\u30d7UP\u30b7\u30ea\u30fc\u30ba, p41\u201348, p163\u2013170 (2024).<\/li> \n
    2. \u50ac\u5947\u6027\u3092\u8efd\u6e1b\u3057\u305f\u8840\u6db2\u304c\u3093\u6cbb\u7642\u306b\u6709\u52b9\u306a\u65b0\u898f\u30b5\u30ea\u30c9\u30de\u30a4\u30c9\u8a98\u5c0e\u4f53\u306e\u958b\u767a. \u5c71\u4e2d \u8061\u58eb. \u533b\u85ac\u54c1\u30e2\u30c0\u30ea\u30c6\u30a3\u306b\u304a\u3051\u308b\u5275\u85ac\u6280\u8853\u958b\u767a\u3068\u5c55\u671b\u3001p227\u2013235\u3001(2024).<\/li> \n
    3. \u6fa4\u5d0e \u9054\u4e5f, \u5c71\u4e2d \u8061\u58eb. \u85ac\u5264\u4f9d\u5b58\u7684\u306b\u30d7\u30ed\u30c6\u30a2\u30bd\u30fc\u30e0\u306b\u3088\u308a\u5206\u89e3\u3055\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u305f\u3081\u306e\u65b0\u6280\u8853. \u751f\u5316\u5b66 \u7b2c95\u5dfb\u7b2c6\u53f7\u3001p736\u2013746\u3001(2023).\n\t\t <\/li> \n\t
    4. \u8fd1\u63a5\u30d3\u30aa\u30c1\u30f3\u5316\u9175\u7d20\u3092\u7528\u3044\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u8a98\u5c0e\u5264\u4f9d\u5b58\u7684\u306a\u76f8\u4e92\u4f5c\u7528\u89e3\u6790\u6280\u8853\u306e\u958b\u767a. \u5c71\u4e2d \u8061\u58eb. \u5317\u9686\u9928:\u300c\u30a2\u30b0\u30ea\u30d0\u30a4\u30aa\u300d\u30016(11)\u3001(2022).\n\t\t <\/li> \n\t\t
    5. \u8fd1\u63a5\u30d3\u30aa\u30c1\u30f3\u5316\u9175\u7d20\u3092\u7528\u3044\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u5206\u89e3\u8a98\u5c0e\u5264\u4f9d\u5b58\u7684\u306a\u76f8\u4e92\u4f5c\u7528\u89e3\u6790\u6280\u8853\u306e\u958b\u767a. \u5c71\u4e2d \u8061\u58eb. \u5317\u9686\u9928\/\u30cb\u30e5\u30fc\u30b5\u30a4\u30a8\u30f3\u30b9\u793e:\u6708\u520a\u300c\u7d30\u80de\u300d\u300154(9)\u3001(2022). \t<\/li> \n\t\t
    6. \u9ad8\u6a4b \u5b8f\u9686, \u5c71\u4e2d \u8061\u58eb. \u30b3\u30e0\u30ae\u7121\u7d30\u80de\u5408\u6210\u7cfb\u306b\u3088\u308b\u8131\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u9175\u7d20\u30a2\u30ec\u30a4\u306e\u4f5c\u88fd\u3068DUB\u963b\u5bb3\u5264\u958b\u767a\u3078\u306e\u5fdc\u7528. \u30d5\u30a1\u30eb\u30de\u30b7\u30a2:\u304f\u3059\u308a\u306e\u79d1\u5b66 (\u65e5\u672c\u85ac\u5b66\u4f1a). 56\u5dfb1\u53f7\u3001p51\u201355\u3001(2020).\n\t\t <\/li> \n\t\t
    7. \u9ad8\u6a4b \u5b8f\u9686, \u5c71\u4e2d \u8061\u58eb, \u5fb3\u6c38\u6587\u7a14, \u6fa4\u5d0e\u9054\u4e5f. \u30d2\u30c8\u8131\u30e6\u30d3\u30ad\u30c1\u30f3\u5316\u9175\u7d20\u30bf\u30f3\u30d1\u30af\u8cea\u30a2\u30ec\u30a4\u306e\u958b\u767a\u3068\u305d\u306e\u5fdc\u7528\u4f8b. \u751f\u5316\u5b66 \u7b2c92\u5dfb\u7b2c1\u53f7\u3001p64\u201374\u3001(2020)<\/li> \n\n<\/div>\n\n\n\n
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