Selected Publications
膜タンパク質生産技術
Yosuke Hashimoto, Wei Zhou, Kohtaroh Hamauchi, Keisuke Shirakura, Takefumi Doi, Kiyohito Yagi, Tatsuya Sawasaki, Yoshiaki Okada, Masuo Kondoh*, Hiroyuki Takeda*. (2018) Engineered membrane protein antigens successfully induce antibodies against extracellular regions of claudin-5. Sci Rep. 8:8383. doi:10.1038/s41598-018-26560-9 Open Access
https://www.nature.com/articles/s41598-018-26560-9
スクリーニング技術
Segawa R, Takeda H, Yokoyama T, et al.
A chalcone derivative suppresses TSLP induction in mice and human keratinocytes through binding to BET family proteins.
Biochem Pharmacol. 2021;194:114819.doi:10.1016/j.bcp.2021.114819
https://www.sciencedirect.com/science/article/abs/pii/S0006295221004354?via%3Dihub
Zhou W, Kaneko N, Nakagita T, Takeda H, Masumoto J.
A comprehensive interaction study provides a potential domain interaction network of human death domain superfamily proteins.
Cell Death Differ. 2021;28(11):2991-3008. doi:10.1038/s41418-021-00796-x
https://www.nature.com/articles/s41418-021-00796-x
原著論文/ Original Papers
Publications in 2021
Morita M, Kanoi BN, Shinzawa N, et al. AGIA Tag System for Ultrastructural Protein Localization Analysis in Blood-Stage Plasmodium falciparum.
Front Cell Infect Microbiol. 2021;11:777291.
doi:10.3389/fcimb.2021.777291
Segawa R, Takeda H, Yokoyama T, et al.
A chalcone derivative suppresses TSLP induction in mice and human keratinocytes through binding to BET family proteins.
Biochem Pharmacol. 2021;194:114819.
doi:10.1016/j.bcp.2021.114819
Ishigami T, Nanki T, Sugawara T, et al.
Rationale and Design of the Orencia Atherosclerosis and Rheumatoid Arthritis Study (ORACLE Arthritis Study): Implications of Biologics against Rheumatoid Arthritis and the Vascular Complications, Subclinical Atherosclerosis.
Methods Protoc. 2021;4(4):83. Open Access
doi:10.3390/mps4040083
Kanako Nishiyama, Masashi Maekawa, Tomoya Nakagita, Jun Nakayama, Takeshi Kiyoi, Mami Chosei, Akari Murakami, Yoshiaki Kamei, Hiroyuki Takeda, Yasutsugu Takada, Shigeki Higashiyama.
CNKSR1 serves as a scaffold to activate an EGFR phosphatase via exclusive interaction with RhoB-GTP.
Life science alliance 4(9) (2021) Open Access
DOI: https://doi.org/10.26508/lsa.202101095
Keisuke Tachibana, Yosuke Hashimoto, Keisuke Shirakura, Yoshiaki Okada, Ryuichi Hirayama, Yumi Iwashita, Itsuki Nishino, Yukio Ago, Hiroyuki Takeda, Hiroki Kuniyasu, Masuo Kondoh.
Safety and efficacy of an anti-claudin-5 monoclonal antibody to increase blood-brain barrier permeability for drug delivery to the brain in a non-human primate.
Journal of controlled release, 336, 105 - 111 (2021)
DOI: 10.1016/j.jconrel.2021.06.0
Wei Zhou, Naoe Kaneko, Tomoya Nakagita, Hiroyuki Takeda*, Junya Masumoto*. （*co-correspondent author)
A comprehensive interaction study provides a potential domain interaction network of human death domain superfamily proteins.
Cell death and differentiation (2021) Open Access
DOI: 10.1038/s41418-021-00796-x
Satoshi Yamanaka, Hidetaka Murai, Daisuke Saito, Gembu Abe, Etsuko Tokunaga, Takahiro Iwasaki, Hirotaka Takahashi, Hiroyuki Takeda, Takayuki Suzuki, Norio Shibata, Koji Tamura, Tatsuya Sawasaki.
Thalidomide and its metabolite 5-hydroxythalidomide induce teratogenicity via the cereblon neosubstrate PLZF.
The EMBO journal 40(4) e105375 (2021) Open Access
DOI: 10.15252/embj.2020105375
Takaaki Yuguchi, Bernard N Kanoi, Hikaru Nagaoka, Toyokazu Miura, Daisuke Ito, Hiroyuki Takeda, Takafumi Tsuboi, Eizo Takashima, Hitoshi Otsuki.
Plasmodium yoelii Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein.
Frontiers in cellular and infection microbiology 11 656620 - 656620 (2021) Open Access
DOI: 10.3791/61871
Publications in 2020
Wei Zhou, Hiroyuki Takeda.
Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins.
Journal of visualized experiments : JoVE (2020)
DOI: 10.3791/61871
Kaneko, N., Kurata, M., Yamamoto, T., Shigemura, T., Agematsu, K., Yamazaki, T., Takeda, H., Sawasaki, T., Koga, T., Kawakami, A., Yachie, A., Migita, K., Yoshiura, K. I., Urano, T., & Masumoto, J.
KN3014, a piperidine-containing small compound, inhibits auto-secretion of IL-1β from PBMCs in a patient with Muckle-Wells syndrome.
Scientific reports, 10(1), 13562. (2020) Open Access
https://doi.org/10.1038/s41598-020-70513-0
Miyoshi S, Tokunaga S, Ozawa T, Takeda H, Aono M, Miyoshi T, Kishi H, Muraguchi A, Shimizu SI, Nozawa A, Sawasaki T. Production of a rabbit monoclonal antibody for highly sensitive detection of citrus mosaic virus and related viruses. PLoS One. 2020 Apr 15;15(4):e0229196. Open Access
doi: 10.1371/journal.pone.0229196.
Publications in 2019
Chigira T, Nagatoishi S, Takeda H, Yoshimaru T, Katagiri T, Tsumoto K.
Biophysical characterization of the breast cancer-related BIG3-PHB2 interaction: Effect of non-conserved loop region of BIG3 on the structure and the interaction. Biochem Biophys Res Commun. 518:183-189. (2020)
doi: 10.1016/j.bbrc.2019.08.028.
Publications in 2018
Yosuke Hashimoto, Wei Zhou, Kohtaroh Hamauchi, Keisuke Shirakura, Takefumi Doi, Kiyohito Yagi, Tatsuya Sawasaki, Yoshiaki Okada, Masuo Kondoh*, Hiroyuki Takeda*. （*co-correspondent author)
Engineered membrane protein antigens successfully induce antibodies against extracellular regions of claudin-5. Sci Rep. 8:8383. (2020)
doi:10.1038/s41598-018-26560-9.
Suzuki Y, Ogasawara T, Tanaka Y, Takeda H, Sawasaki T, Mogi M, Liu S, Maeyama K. Functional G-Protein-Coupled Receptor (GPCR) Synthesis: The Pharmacological Analysis of Human Histamine H1 Receptor (HRH1) Synthesized by a Wheat Germ Cell-Free Protein Synthesis System Combined with Asolectin Glycerosomes. Front Pharmacol. 2018 Feb 6;9:38. doi: 10.3389/fphar.2018.00038.
Uematsu A, Kido K, Manabe E, Takeda H, Takahashi H, Hayashi M, Imai Y, Sawasaki T. DANFIN functions as an inhibitor of transcription factor NF-κB and potentiates the antitumor effect of bortezomib in multiple myeloma. Biochem Biophys Res Commun. 2018 Jan 15;495(3):2289-2295. doi: 10.1016/j.bbrc.2017.12.142. Epub 2017 Dec 25. PubMed PMID: 29284118.
Publications in 2017
Liu S, Hasegawa H, Takemasa E, Suzuki Y, Oka K, Kiyoi T, Takeda H, Ogasawara T, Sawasaki T, Yasukawa M, Maeyama K. Efficiency and Safety of CRAC Inhibitors in Human Rheumatoid Arthritis Xenograft Models. J Immunol. 2017 Jul 17. pii: ji1700192. doi: 10.4049/jimmunol.1700192.
Kaneko N, Ito Y, Iwasaki T, Takeda H, Sawasaki T, Migita K, Agematsu K, Koga T, Kawakami A, Yachie A, Yoshiura K, Morikawa S, Kurata M, Masumoto J. (2017)
Poly (I:C) and hyaluronic acid directly interact with NLRP3, resulting in the assembly of NLRP3 and ASC in a cell-free system. European Journal of Inflammation. DOI: https://doi.org/10.1177/1721727X17711047. Open Access
Takeda H, Zhou W, Kido K, Suno R, Iwasaki T, Kobayashi T, Sawasaki T. (2017) CP5 system, for simple and highly efficient protein purification with a C-terminal designed mini tag. PLoS One. 12:e0178246. doi: 10.1371/journal.pone.0178246. Open Access
Kaneko N, Iwasaki T, Ito Y, Takeda H, Sawasaki T, Morikawa S, Nakano N, Kurata M, Masumoto J. Applications of reconstituted inflammasomes in a cell-free system to drug discovery and elucidation of the pathogenesis of autoinflammatory diseases. Inflammation and Regeneration201737:9 DOI: 10.1186/s41232-017-0040-y Open Access
Yamamoto T, Taira Nihira N, Yogosawa S, Aoki K, Takeda H, Sawasaki T, Yoshida K. Interaction between RNF8 and DYRK2 is required for the recruitment of DNA repair molecules to DNA double-strand breaks. FEBS Lett. 2017 Mar;591(6):842-853. doi: 10.1002/1873-3468.12596.
Publications in 2016
Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, Tokunaga F. (2016) Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 7:12547. doi: 10.1038/ncomms12547.
Iwasaki T, Kaneko N, Ito Y, Takeda H, Sawasaki T, Heike T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J. (2016) Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset Sarcoidosis. ScientificWorldJournal. 2597376. doi: 10.1155/2016/2597376.
Yano T, Takeda H, Uematsu A, Yamanaka S, Nomura S, Nemoto K, Iwasaki T, Takahashi H, Sawasaki T. (2016) AGIA Tag System Based on a High Affinity Rabbit Monoclonal Antibody against Human Dopamine Receptor D1 for Protein Analysis. PLoS One. 11:e0156716. doi: 10.1371/journal.pone.0156716. Open Access
Takahashi H, Uematsu A, Yamanaka S, Imamura M, Nakajima T, Doi K, Yasuoka S, Takahashi C, Takeda H, Sawasaki T. (2016) Establishment of a Wheat Cell-Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins. PLoS One. 11:e0156718. doi: 10.1371/journal.pone.0156718. Open Access
Publications in 2015
Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T. (2015) Production of monoclonal antibodies against GPCR using cell-free synthesized GPCR antigen and biotinylated liposome-based interaction assay. Sci Rep. 5:11333. Open Access
Ramadan A, Nemoto K, Seki M, Shinozaki K, Takeda H, Takahashi H, Sawasaki T. (2015) Wheat germ-based protein libraries for the functional characterisation of the Arabidopsis E2 ubiquitin conjugating enzymes and the RING-type E3 ubiquitin ligase enzymes. BMC Plant Biol. 15:275.
Kaneko N, Ito Y, Iwasaki T, Takeda H, Sawasaki T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J (2015) Reconstituted AIM2 inflammasome in cell-free system. J Immunol Methods 426:76-81.
Ohnishi T, Yanazawa M, Sasahara T, Kitamura Y, Hiroaki H, Fukazawa Y, Kii I, Nishiyama T, Kakita A, Takeda H, Takeuchi A, Arai Y, Ito A, Komura H, Hirao H, Satomura K, Inoue M, Muramatsu SI, Matsui K, Tada M, Sato M, Saijo E, Shigemitsu Y, Sakai S, Umetsu Y, Goda N, Takino N, Takahashi H, Hagiwara M, Sawasaki T, Iwasaki G, Nakamura Y, Nabeshima YI, Teplow DB, Hoshi M. (2015) Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. Proc Natl Acad Sci U S A. 112:E4465-74.
Nakajia M, Nagase S, Iida M, Takeda S, Yamashita M, Watari A, Shirasago Y, Fukasawa M, Takeda H, Sawasaki T, Yagi K, Kondoh M. (2015) Claudin-1 binder enhances epidermal permeability in a human keratinocyte model. J Pharmacol Exp Ther. 354:440-447.
Onishi S, Adnan E, Ishizaki J, Miyazaki T, Tanaka Y, Matsumoto T, Suemori K, Shudou M, Okura T, Takeda H, Sawasaki T, Yasukawa M, Hasegawa H. (2015) Novel Autoantigens Associated with Lupus Nephritis. PLoS One. 10:e0126564. 2014
Publications in 2014
Arimitsu E, Ogasawara T, Takeda H, Sawasaki T, Ikeda Y, Hiasa Y, Maeyama K. (2014) The ligand binding ability of dopamine D1 receptors synthesized using a wheat germ cell-free protein synthesis system with liposomes. Eur J Pharmacol. 745:117-122.
Takemori N, Takemori A, Matsuoka K, Morishita R, Matsushita N, Aoshima M, Takeda H, Sawasaki T, Endo Y, Higashiyama S. (2014) High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system. Mol Biosyst. 11:361-365.
Mizutani Y, Tsuge S, Takeda H, Hasegawa Y, Shiogama K, Onouchi T, Inada K, Sawasaki T, Tsutsumi Y. (2014) In situ visualization of plasma cells producing antibodies reactive to Porphyromonas gingivalis in periodontitis: the application of the enzyme-labeled antigen method. Mol Oral Microbiol. 29:156-73.
Nagayoshi Y, Nakamura M, Matsuoka K, Ohtsuka T, Mori Y, Kono H, Aso T, Ideno N, Takahata S, Ryo A, Takeda H, Ito T, Oda Y, Endo Y, Sawasaki T, Tanaka M. (2014) Profiling of autoantibodies in sera of pancreatic cancer patients. Ann Surg Oncol. Suppl 3:S459-465.
Takiuchi T, Nakagawa T, Tamiya H, Fujita H, Sasaki Y, Saeki Y, Takeda H, Sawasaki T, Buchberger A, Kimura T, Iwai K. (2014) Suppression of LUBAC-mediated linear ubiquitination by a specific interaction between LUBAC and the deubiquitinases CYLD and OTULIN. Genes Cells. 19:254-272.
2013
Ishigami T, Abe K, Aoki I, Minegishi S, Ryo A, Matsunaga S, Matsuoka K, Takeda H, Sawasaki T, Umemura S, Endo Y. (2013) Anti-interleukin-5 and multiple autoantibodies are associated with human atherosclerotic diseases and serum interleukin-5 levels. FASEB J. 27:3437-3445.
Mizutani Y, Matsuoka K, Takeda H, Shiogama K, Inada K, Hayakawa K, Yamada H, Miyazaki T, Sawasaki T, Endo Y, Tsutsumi Y. (2013) Novel approach to identifying autoantibodies in rheumatoid synovitis with a biotinylated human autoantigen library and the enzyme-labeled antigen method. J Immunol Methods. 387:57-70.
2012
Takahashi H, Ozawa A, Nemoto K, Nozawa A, Seki M, Shinozaki K, Takeda H, Endo Y, Sawasaki T. (2012) Genome-wide biochemical analysis of Arabidopsis protein phosphatase using a wheat cell-free system. FEBS Lett. 586:3134-3141.
2011
Takeda H, Yoneyama F, Kawai S, Hashimoto W, and Murata K. (2011) Bioethanol production from marine biomass alginate by metabolically engineered bacteria. Ener Environ Sci 4 2575 - 2581.
before 2010
Takeda H, Kawamura Y, Miura A, Mori M, Wakamatsu A, Yamamoto J, Isogai T, Matsumoto M, Nakayama K, Natsume T, Goshima N and Nomura N. (2010) Comparative Analysis of Human Src-Family Kinase substrate specificity in vitro. J Proteome Res 9 5982 - 5993.
Takeda H, Sugahara T, Kotake T, Nakagawa N and Sakurai N. (2010) Sugar treatment inhibits IAA-induced expression of endo-1,3:1,4-β-glucanase EI transcripts in barley coleoptile segments. Physiol Plantarum 139, 413-420.
Goshima N, Kawamura Y, Fukumoto A, Miura A, Honma R, Satoh R, Wakamatsu A, Yamamoto J,Kimura K, Nishikawa T, Andoh T, Iida Y, Ishikawa K, Ito E, Kagawa N, Kaminaga C, Kanehori K,Kawakami B, Kenmochi K, Kimura R, Kobayashi M, Kuroita T, Kuwayama H, Maruyama Y, Matsuo K,Minami K, Mitsubori M, Mori M, Morishita R, Murase A, Nishikawa A, Nishikawa S, Okamoto T,Sakagami N, Sakamoto Y, Sasaki Y, Seki T, Sono S, Sugiyama A, Sumiya T, Takayama T, Takayama Y, Takeda H, Togashi T, Yahata K, Yamada H, Yanagisawa Y, Endo Y, Imamoto F, Kisu Y, Tanaka S, Isogai T, Imai J, Watanabe S, Nomura N. (2008) Human Protein Factory: an infrastructure to convert the human transcriptome into the in vitro-expressed human proteome of versatile utility. Nat Methods 5, 1011-1017.
Takeda H, Ueno A, Miura A, Goshima N and Nomura N. (2006) High-throughput kinase assay based on surface plasmon resonance suitable for native protein substrates. Anal Biochem 357, 262-71.
Takeda H, Yoshikawa T, Liu XZ, Nakagawa N, Li YQ and Sakurai N. (2004) Molecular cloning of two exo-β-glucanases and their in vivo substrates in the cell walls of lily pollen tubes. Plant Cell Physiol 45, 436-444.
Zhou W, Takeda H, Liu XZ, Nakagawa N, Sakurai N, Huang J and Li YQ. (2004) A novel endo-1,4-β-glucanase gene (LlpCel1) is exclusively expressed in pollen and pollen tubes of Lilium longiflorum. Acta Bot Sin 46, 142-147.
Takeda H, Kotake T, Nakagawa N, Sakurai N and Nevins DJ (2003) Expression and function of cell wall-bound cationic peroxidase in asparagus somatic embryogenesis. Plant Physioly 131, 1765-1774.
総説/ Reviews
竹田浩之. (2022) Alpha技術によるHTS. 実験医学別冊：創薬研究のためのスクリーニング学実践テキスト〜アッセイ系の選択・構築から、ヒット・リード化合物の同定、自動化まで. スクリーニング学研究会／編 羊土社 ISBN 978-4-7581-2258-0
Zhou W, Takeda H. (2018) Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System. Shuang Liu (ed.), Rheumatoid Arthritis: Methods and Protocols, Methods in Molecular Biology 1868:49-67. doi: 10.1007/978-1-4939-8802-0_7. PubMed PMID: 30244454.
Takeda H. (2018) Autoantibody Profiling Using Human Autoantigen Protein Array and AlphaScreen. Shuang Liu (ed.), Rheumatoid Arthritis: Methods and Protocols, Methods in Molecular Biology 1868:49-67. doi: 10.1007/978-1-4939-8802-0_10. PubMed PMID: 30244457.
竹田浩之、澤崎達也 （2017）AGIAタグシステム: 細胞生物学研究に最適な高感度検出およびキャプチャー用ペプチドタグ. 生化学 89, 302-307, 社団法人日本生化学会
竹田浩之、澤崎達也 （2016）無細胞技術を基盤とした抗GPCR モノクローナル抗体作製技術の開発. 月刊BIOINDUSTRY, 株式会社シーエムシー出版
竹田浩之、野澤彰、澤崎達也 (2015) リポソーム添加型コムギ無細胞合成系による膜タンパク質合成とその利用.ファルマシア 51, 770-774, 日本薬学会
竹田浩之、澤崎達也 自己抗体の新たな探索法 (2014) 炎症と免疫 22, 479-484. 先端医学社
澤崎達也、竹田浩之、高橋宏隆、根本圭一郎，無細胞タンパク質アレイを用いたプロテインキナーゼおよびユビキチンリガーゼの基質タンパク質探索技術（2013）生化学 85，438-446，社団法人日本生化学会
Takeda H, Goshima N and Nomura N. (2010) High-throughput kinase assay based on surface plasmon resonance. de Mol NJ and Fischer MJE (eds.), Surface Plasmon Resonance, Methods in Molecular Biology 627, Humana Press, pp. 131-145.
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