研究業績 

2014年

　原著論文

• Nomura Y, Izumi A, Fukunaga Y, Kusumi K, Iba K, Watanabe S, Nakahira Y, Weber AMP, Nozawa A, Tozawa Y. Diversity in Guanosine 3',5'-Bisdiphosphate (ppGpp) Sensitivity Among Guanylate Kinases of Bacteria and Plants. J. Biol. Chem. 289:15631-15641 (2014)
• Nomura Y, Nozawa A, Tozawa Y. Biochemical analyses of ppGpp effect on adenylosuccinate synthetases, key enzymes in purine biosynthesis in rice. Biosci. Biotechnol. Biochem. (2014) in press
• Nozawa A, Tozawa Y. Incorporation of adenosine nucleotide transporter, ANT1, into proteoliposomes facilitates ATP translocation and activation of encapsulated luciferase. J. Biosci. Bioeng. S1389-1723(14)00035-8. (2014)
• Watanabe S, Tanimoto Y, Yamauchi S, Tozawa Y, Sawayama S, Watanabe Y. Identification and Characterization of trans-3-Hydroxy-L-proline Dehydratase and <DELTA>¹-Pyrroline-2-carboxylate Reductase Involved in trans-3-Hydroxy-L-proline Metabolism of Bacteria. FEBS Open Bio 4:240-250 (2014)

　総説・解説 

• 野澤彰，戸澤譲．膜輸送タンパク質の完全インビトロ機能解析系．バイオサイエンスとインダストリー. 72:(3) 190-196 (2014) 
• Nozawa A, Tozawa Y. Modifications of wheat germ cell-free system for functional proteomics of plant membrane proteins. Methods in Molecular Biology. Springer Science+Business Media, LLC, New York, NY. 1072: 259-272 (2014)  

2013年

　原著論文

• Nakahira Y, Ogawa A, Asano H, Oyama T, Tozawa Y. Theophylline-dependent Riboswitch as a Novel Genetic Tool for Strict Regulation of Protein Expression in Cyanobacterium Synechococcus elongatus PCC7942. Plant Cell Physiol. 54:1724-1735 (2013)
• Nakahira Y, Ishikawa K, Tanaka k, Tozawa Y, Shiina T. Overproduction of hyperthermostable β-1,4-endoglucanase from the archaeon Pyrococcus horikoshii by tobacco chloroplast engineering. Biosci. Biotechnol. Biochem. 77: 2140-2143 (2013)
• Kore-eda S, Nozawa A, Okada Y, Nishiyama Y, Tozawa Y. Characterization of phosphoenolpyruvate/phosphate translocator from iceplant. Biosci. Biotechnol. Biochem. 77:1511-1516 (2013)
• Kubota S, Shirai O, Hibi T, Tozawa Y, Kano K. Effect of counter ions on the transport current across membranes containing KAT1 potassium channel. Analytical Sciences 29:161-164 (2013)

　総説・解説 

• Bernhard F, Tozawa Y. Cell-Free Expression - Making a Mark. Curr. Opin. Struct. Biol. 23: 374-380 (2013)

2012年

　原著論文

• Watanabe S, Morimoto D, Fukumori F, Shinomiya H, Nishiwaki H, Kawano-Kawada M, Sasaki Y, Tozawa Y, Watanabe Y. Identification and characterization of D-Hydroxyproline dehydrogenase and Δ1-Pyrroline-4-Hydroxy-2-Carboxylate Deaminase involved in novel L-Hydroxyproline metabolism of bacteria: METABOLIC CONVERGENT EVOLUTION. J. Biol. Chem. 283:32674-32688 (2012)
  
• Kato H, Watanabe S, Nimura-Matsune K, Chibazakura T, Tozawa Y, Yoshikawa H. Exploration of the possible partnership amoung orphan two-component system proteins in cyanobacterium Synechococcus elongates PCC 7942. Biosci. Biotechnol. Biochem. 76:1484-1491(2012)
  
• Yagi Y, Ishizaki Y, Nakahira Y, Tozawa Y, Shiina T. Non-prokaryotic nucleoid protein pTAC3 is essential for transcription by a cyanobacteria-derived RNA polymerase in chloroplasts. Proc. Natl. Acad. Sci. USA 109:7541-7546 (2012) 
  
• Tagami K, Nanamiya H, Kazo Y, Maehashi M, Suzuki S, Namba E, Tozawa Y, Morimoto T, Ogasawara N, Kageyama Y, Ara K, Ozaki K, Yoshida M, Kuroiwa T, Ohashi Y, Kawamura F. Expression of small (p)ppGpp synthetase, YwaC, in the (p)ppGpp0 mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome. MicrobiologyOpen 1:115-134 (2012) 
  
• Nomura Y, Takabayashi T, Kuroda H, Yukawa Y, Sattasuk K, Akita M, Nozawa A, Tozawa Y. ppGpp inhibits peptide elongation cycle of chloroplast translation system in vitro. Plant Mol. Biol. 78:185-196 (2012) 

　総説・解説・プロシーディングス 

• Tozawa Y. Cell-free protein synthesis of membrane-integrated proteins for functional analysis. (2011) Frontiers in Agriculture Proteome Research, Contribution of Proteomics Technology in Agricultural Sciences, Proceedings of the 3rd International Symposium on “Frontiers in Agriculture Proteome Research”, Eds., Mock HP, Wang ZY, and Komatsu S. 16-23 (2012)   

2011年   

　原著論文 

• Sattasuk K, Nozawa A, Tozawa Y, Kakinuma Y, Akita M. In vitro protein import of a putative amino acid transporter from Arabidopsis thaliana into chloroplasts and its suborganellar localization. Biosci. Biotechnol. Biochem. 75:2200-2006 (2011) 
  
• Nozawa A, Fujimoto R, Matsuoka H, Tsuboi T, Tozawa Y. Cell-free protein synthesis, reconstitution and characterization of mitochondrial dicarboxylate-tricarboxylate carrier of Plasmodium falciparum. Biochem. Biophys. Res. Commun. 414:612-617 (2011) 
  
• Tozawa Y, Nomura Y. Signaling by the global regulatory molecule ppGpp in bacteria and chloroplasts of land plants. Plant Biol. 13:609-709 (2011) 
  
• Narimatsu Y, Kubota T, Furukawa S, Shimojima M, Iwasaki H, Tozawa Y, Tachibana K, Narimatsu H. Co-translational function of Cocmc, core 1 synthase specific molecular chaperine, revealed by a cell-free translation system. FEBS Lett. 585:1276-1280 (2011) 
  
• Ito K, Nozaki M, Ohta T, Miura C, Tozawa Y, Miura T. Differences of two polychaete species reflected in enzyme activities. Marine Biol. 158:1211-1221 (2011) 
  
• Ohta T, Ueda Y, Ito K, Celino FT, Miura C, Yamashita H, Miura T, Tozawa Y. Anti-viral effects of interferon administration on Sevenband grouper, Epinephelus septemfasciatus. Fish Shellfish Immunol. 30:1064-1071 (2011)  
  
• Celino FT, Yamaguchi S, Miura C, Ohta T, Tozawa Y, Iwai T, Miura T. Tolerance of spermatogonia to oxidatve stress is due to high levels of Zn and Cu/Zn superoxide dismutase. PLoS ONE 6:e16938 (2011) 

2010年   

　原著論文 

• Genji T, Nozawa A, and Tozawa Y. Efficient production and purification of functional bacteriorhodopsin with a wheat-germ cell-free system and a combination of Fos-choline and CHAPS detergents. Biochem. Biophys. Res. Commun. 400:638-642 (2010) 
  
• Chadani Y, Ono K, Ozawa S-I, Takahashi Y, Takai K, Nanamiya H, Tozawa Y, Kutsukake K, and Abo T. Ribosome rescue by Escherichia coli AffA (YhdL) in the absence of trans-translation system. Mol. Microbiol. 78:796-808 (2010) 
  
• Yamauchi S, Fusada N, Hayashi H, Utsumi T, Uozumi N, Endo Y, and Tozawa Y. The concensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system. FEBS J. 277:3596-3607 (2010) 
  
• Nishiyama Y, Yun CS, Masuda F, Sasaki T, Saito K, and Tozawa Y. Expression of bacterial tyrosine ammonia-lyase creates a novel p-coumaric acid pathway in the biosynthesis of phenylpropanoids in Arabidopsis thaliana. Planta 232 (1):201-218 (2010) 
  
• Ohta T, Matsuoka H, Nomura Y, and Tozawa Y. Control of translational initiation in the wheat-embryo cell-free protein expression system for producing homogenous products. Protein Expr. Purif. 73:15-22 (2010) 

　総説・解説 

• 戸澤譲，笠井光治．高等植物におけるppGpp合成系とその生理学的機能の解明をめざして. 植物の生長調節 45(2):119-124 (2010)  
  
• Nozawa A, Nanamiya H, and Tozawa Y. Production of membrane proteins through the wheat-germ cell-free technology. Methods in Molecular Biology. Humana Press Inc., Totowa, NJ. 607:213-218 (2010) 
  
• Kanno T, and Tozawa Y. Protein engineering accelerated by cell-free technology. Methods in Molecular Biology. Humana Press Inc., Totowa, NJ. 607:85-99 (2010)   

2009年   

　原著論文 

• Natori Y, Tagami K, Murakami K, Yoshida S, Tanigawa O, Masuda K, Wada T, Suzuki S, Nanamiya H, Tozawa Y, and Kawamura F. Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient of (p)ppGpp synthetase genes, relA, yjbM, and ywaC. J. Bacteriol.191 (14):4555-4561 (2009) 
  
• Nozaki M, Miura C, Tozawa Y, and Miura T. The contribution of endogenous-cellulose digestion in the gut of earthworm (Pheretima hilgendorfi:Megascolecidae). Soil Biol. Biochem. 41:762-769 (2009) 
  
• Yun CS, Hasegawa H, Terakawa T, and Tozawa Y. Novel bacterial N-acetyltransferase gene for herbicide detoxification in land plants and selectable marker in plant transformation. Biosci. Biotechnol. Biochem. 73(5):1000-1006 (2009) 

　総説・解説 

• 野澤彰，戸澤譲，澤崎達也，遠藤弥重太．コムギ無細胞翻訳系を用いた膜タンパク質生産．蛋白質核酸酵素. 54:(12) 1443-1447 (2009) 
  
• 戸澤譲，野澤彰，源治尚久．無細胞翻訳系を利用した膜タンパク質の機能解析．化学と生物．47:(2) 98-103 (2009)   

2008年 

　原著論文 

• Masuda S, Tozawa Y, and Ohta H. Possible targets of "magic spots" in plant signaling. Plant Signaling & Behavior 3 (11): 1021-1023 (2008) 
  
• Yamada T, Matsuda F, Kasai K, Fukuoka S, Kitamura K, Tozawa Y, Miyagawa H, and Wakasa K. Mutation of an enzyme that functions in phenylalanine biosynthesis results in accumulation of phenylalanine and tryptophan in rice. Plant Cell 20 (5):1316-1329 (2008) 
  
• Yun CS, Yamamoto T, Nozawa A, Tozawa Y. Expression of parsley flavone synthase I establishes the flavone biosynthetic pathway in Arabidopsis thaliana. Biosci. Biotechnol. Biochem.72 (4): 968-973 (2008) 
  
• Masuda S, Mizusawa K, Narisawa T, Tozawa Y, Ohta H, and Takamiya KI. The Bacterial Stringent Response, Conserved in Chloroplasts, Controls Plant Fertilization. Plant Cell Physiol. 49 (2):135-141 (2008) 
  
• Nanamiya H, Kasai K, Nozawa A, Yun C-S, Narisawa T, Murakami K, Natori Y, Kawamura F, and Tozawa Y. Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis. Mol. Microbiol. 67 (2): 291-304 (2008) 

　総説・解説・プロシーディングス 

• 戸澤譲，笠井光治，七宮英晃．高等植物とバクテリアにおける多様なppGpp合成系とその生理学的機能．バイオサイエンスとインダストリー 66:293-297 (2008) 
• Nozaki M, Miura C, Tozawa Y, and Miura T. The role of the earthworm, Pheretima (Metaphire) holgendorfi, in terrestrial ecosystem nutrient cycling. Interdisciplinary Studies on Environmental Chemistry–Biological Responses to Chemical Pollutants, Eds., Murakami Y, Nakayama N, Kitamura S-I, Iwata H, and Tanabe S. 275-279 (2008)
• Tagami K, Wada T, Maehashi M, Hirohata Y, Yoshida M, Kuroiwa H, Kuroiwa T, Nanamiya H, Tozawa Y, Liu S, Kageyama Y, Ara K, Ozaki K, Kawamura F. Functional analysis of a novel ppGpp synthetase, YwaC, and regulatory mechanism for the dimerization of ribosome in Bacillus subtilis. Genes & Genetics Systems 83:513 (2008)
• Tagami K, Wada T, Yoshida M, Kuroiwa H, Kuroiwa T, Nanamiya H, Tozawa Y, Kawamura F. Functional analysis of a novel ppGpp synthetase, YwaC, in Bacillus subtilis. Genes & Genetics Systems 83:513 (2008)  

2007年 

　原著論文 

• Nozawa A, Nanamiya H, Mityata T, Linka N, Endo Y, Weber APM, and Tozawa Y. A cell-free translation and proteoliposome-reconstitution system for functional analysis of plant solute transporters. Plant Cell Physiol. 48 (12): 1815-1820 (2007) 
  
• Tozawa Y, Nozawa A, Kanno T, Narisawa T, Masuda S, Kasai K, Nanamiya, H. Calcium-activated (p)ppGpp synthetase in chloroplasts of land plants. J. Biol. Chem. 282 (49): 35536-35545 (2007) 
  
• Tozawa Y, Teraishi M, Sasaki T, Sonoike K, Nishiyama Y, Itaya M, Miyao A, and Hirochika H. The plastid sigma factor SIG1 maintains photosystem I activity via regulated expression of the psaA operon in rice chloroplasts. Plant J. 52: 124-132 (2007) 
  
• Kojima K, Oshita M, Nanjo Y, Kasai K, Tozawa Y, Hayashi H, and Nishiyama Y. Oxidation of elongation factor G inhibits the synthesis of the D1 protein of photosystem II. Mol. Microbiol. 64: 936-947 (2007) 
  
• Kubota Y, Miyao A, Hirochika H, Tozawa Y, Yasuda H, Tsunoyama Y, Niwa Y, Imamura S, Shirai M, and Asayama M. Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase in rice: tissue-specific and light-responsive gene expression. Plant Cell Physiol.48: 186-192 (2007)  
  
• Kanno T, Kitano M, Kato R, Ohmori A, Endo Y, and Tozawa Y. Sequence specificity and efficiency of protein N-terminal methionine elimination in wheat-embryo cell-free system. Protein Expr. Purif. 52: 59-65 (2007)  
  
• Kuroita T, Kanno T, Kawai A, Kawakami B, Oka M, Endo Y, and Tozawa Y. Functional similarities of a thermostable protein-disulfide oxidoreductase identified in the archaeon Pyrococcus horikoshii to bacterial DsbA enzymes. Extremophiles 11: 85-94 (2007)    

　プロシーディングス

• Kasai K, Obayashi Y, Yamada T, Kanno T, Wakasa K, Tozawa Y. Functional analysis of arogenate dehydratase of rice by means of wheat-embryo cell-free protein expression system. Plant Cell Physiol.48:S219 (2007)
• Tozawa Y, Teraishi M, Sasaki T, Sonoike K. Functional roles of plastid SIG1 for gene expression of photosystem I components. Plant Cell Physiol.48:S35 (2007) 

2006年 

　原著論文 

• Wakasa K, Hasegawa H, Nemoto H, Matusda F, Miyazawa H, Tozawa Y, Morino K, Komatsu A, Yamada T, Terakawa T, Miyagawa H. High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. Journal of Experimental Botany 57: 3069-3078 (2006) 
  
• Ozaki Y, Higuchi M, Miura C, Yamaguchi S, Tozawa Y, and Miura T. Roles of 11b-hydroxysteroid dehydrogenase in fish spermatogenesis. Endocrinology 147: 5139-5146(2006)  
  
• Makino SI, Sawasaki T, Tozawa Y, Endo Y, and Takai K. Covalent circularization of exogenous RNA during incubation with a wheat embryo cell extract. Biochem. Biophys. Res. Commun. 347: 1080-1087 (2006) 
  
• Hirano N, Sawasaki T, Tozawa Y, Endo Y, and Takai K. Tolerance for random recombination of domains in prokaryotic and eukaryotic translation systems: limited inter-domain misfolding in a eukaryotic translation system. Proteins 64: 343-354 (2006)   

　プロシーディングス

• Murakami K, Natori Y, Sato M, Nanamiya H, Kawamura F, Tozawa Y. Function analysis of relA and it’s paralogous genes, yjbM and ywaC, in Bacillus subtilis. Genes & Genetics Systems 81:442 (2006)  

2005年 

　原著論文 

• Kanno T, Komatsu A, Kasai K, Dubouzet JG, Sakurai M, Ikejiri-K Y, Wakasa K, and Tozawa Y. Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant Trp biosynthetic pathway. Plant Physiol. 138: 2260-2268 (2005) 
  
• Kasai K, Kanno T, Akita M, Ikejiri-Kanno Y, Wakasa K, and Tozawa Y. Identification of three shikimate kinase genes in rice: characterization of their differential expression during panicle development and of the enzymatic activities of the encoded proteins. Planta 222:438-447 (2005) 

　総説・解説  

• 戸澤譲．進化する無細胞タンパク質合成技術．BIONICS. MAY 2005: 44-49 (2005) 
  
• 戸澤譲，越智幸三．葉緑体(p)ppGpp合成酵素．蛋白質核酸酵素. 50:(14) 1860 (2005) 
  
• Sawasaki T, Gouda MD, Kawasaki T, Tsuboi T, Tozawa Y, Takai K, and Endo Y. The Wheat Germ Cell-Free Expression System. Methods in Molecular Biology. Humana Press Inc., Totowa, NJ. 310:131-144 (2005)   

2004年 

　原著論文 

• Kasai K, Kanno T, Wakasa K, Endo Y, and Tozawa Y. Guanosine tetra- and pentaphosphate synthetase activity in chloroplasts of a higher plant: association with 70S ribosomes and inhibition by tetracycline. Nucleic Acids Res. 32: (19) 5732-5741 (2004)  
  
• Kusumi K, Yara A, Mitsui N, Tozawa Y, and Iba K. Characterization of a rice nuclear-encoded plastid RNA polymerase gene OsRpoTp. Plant Cell Physiol. 45: (9) 1194-1201 (2004)  
  
• Yamada T, Tozawa Y, Hasegawa H, Terakawa T, Ohkawa Y, and Wakasa K. Use of a feedback-insensitive a subunit of anthranilate synthase as a selectable marker for transformation of rice and potato. Molecular Breeding 14: 363-373 (2004) 
  
• Sugimoto H, Kusumi K, Tozawa Y, Yazaki J, Kishimoto N, Kikuchi S, and Iba K. The virescent-2 Mutation Inhibits Translation of Plastid Transcripts for the Plastid Genetic System at an early stage of chloroplast differentiation. Plant Cell Physiol. 45: (8)985-996 (2004) 
  
• Tanaka N, Fujita M, Handa H, Muryama S, Umemura M, Kawamura Y, Mitsui T, Mikami S, Tozawa Y, Yoshinaga T, and Komatsu S. Proteomics of the rice cell: systematic identification of the protein population in subcellular compartments. Mol. Genet. Genomics 271: (5) 566-576 (2004) 
  
• Kanno T, Kasai K, Ikejiri-Kanno Y, Wakasa K, and Tozawa Y. In vitro reconstitution of rice anthranilate synthase: distinct functional properties of the a subunits OASA1 and OASA2. Plant Mol. Biol. 54: (1) 11-23 (2004)  
  
• Kasai K, K-Kobayashi M, Teraishi M, Ito Y, Ochi K, Wakasa K, and Tozawa Y. Differential Expression of Three Plastidial Sigma Factors, OsSIG1, OsSIG2A and OsSIG2B, during leaf development in rice. Biosci Biotechnol Biochem. 68: (4) 973-977 (2004) 

　総説・解説・プロシーディングス

• Ochi K, Okamoto S, Tozawa Y, Inaoka T, Hosaka T, Xu J, and Kurosawa K. Ribosome Engineering and Secondary Metabolite Production. Advances in Applied Microbiology. Academic Press, Review 56: 155- 184 (2004) 
• Sugimoto H, Kusumi K, Yoshimura A, Kikuchi S, Tozawa Y, Iba K. Functional analysis of rice virescent-2 gene essential for chloroplast development. Plant Cell Physiol. 45:S208 (2004)   

2003年 

　原著論文 

• Ohashi Y, Inaoka T, Kasai K, Ito Y, Okamoto S, Satsu H, Tozawa Y, Kawamura F, and Ochi K. Expression Profiling of Translation-associated Genes in Sporulation Bacillus subtilis and Consequence of Sporulation by Gene Inactivation. Biosci Biotechnol Biochem. 67: (10) 2245-2253 (2003) 

　総説・解説・プロシーディングス  

• 若狭暁、戸澤譲. トリプトファン生合成における一次・二次代謝の制御と利用をめざして. 植物の生長調節38:249-254 (2003) 
  
• Tozawa Y. “Magic spot” synthase in chloroplasts. 日本生化学会誌 ミニレビュー 75:600-604 (2003) 
  
• Tozawa Y, Sawasaki T, and Endo Y. Cell-free Protein Synthesis by Wheat Germ Extracts. 日本結晶学会誌 総説, 45:3-8 (2003)
• Kusumi K, Kouno T, Sugimoto H, Tozawa Y, Yoshimura A, Kikuchi S, Iba K. Expression analysis of chloroplast proteins in virescent mutants (v(1), v(3)) of rice. Plant Cell Physiol. 44:S57 (2003)    

2002年まで 

　原著論文 

• Ito Y, Ozawa A, Sawasaki T, Endo Y, Ochi K, and Tozawa Y. OsRALyase1, a Putative F-Box Protein Identified in Rice Oryza sativa, Possesses Enzyme Activity Identical to That of Wheat RALyase. Biosci Biotechnol Biochem. 66: 2725-2729 (2002) 
  
• Kasai K, Usami S, Yamada T, Endo Y, Ochi K, and Tozawa Y. A RelA-SpoT homolog (Cr-RSH) identified in Chlamydomonas reinhardtii generates stringent factor in vivo and localizes to chloroplasts in vitro. Nucleic Acids Res. 30: 4985-4992 (2002) 
  
• Xu J, Tozawa Y, Lai C, Hyashi H, and Ochi K. Rifampicin resistant mutation in the rpoB gene confers ppGpp-independent antibiotic production to Streptomyces coelicolor A3(2). Mol. Gen. Genet. 268: 179-189 (2002)  
  
• Lai C, Xu J, Tozawa Y, Okamoto-H Y, Yao X, and Ochi K. Genetic and physiological characterization of rpoB mutations which activate antibiotic production in Streptomyces lividans. Microbiology 148: 3365-3373 (2002)  
  
• Tozawa Y, Hasegawa H, Terakawa T, and Wakasa K. Characterization of rice anthranilate synthase alfa subunit genes OSASA1 and OSASA2: tryptophan accumulation in transgenic rice expressing a feedback-insensitive mutant of OASA1. Plant Physiol. 126: 1493-1506 (2001) 
  
• Urushibara S, Tozawa Y, Kawagishi-Kobayashi M, and Wakasa K. Efficient transformation of rice suspension-cultured cells mediated by Agrobacterium tumefaciens. Breeding Science 51: 33-38 (2001) 
  
• Asada A, Zhao Y, Komano H, Kuwata T, Mukai M, Fujita K, Tozawa Y, Iseki R, Tian H, Sato K, Motegi K, Suzuki R, Yokoyama M, and Iwata M. The calcium-independent protein kinase C participates in an early process of CD3/CD28-mediated induction of thymocyte apoptosis. Immunology 101: 309-315 (2000)  
  
• Tsunoyama Y, Tozawa Y, and Wakasa K. Coexistence of the plastidial sigma factor SigA and bacterial RNA polymerase activity in partially purified chloroplast soluble protein fraction. Rice Genet. Newslett. 15:176-178 (1999) 
  
• Tozawa Y, Tanaka K, Takahashi H, and Wakasa K. Nuclear encoding of a plastid sigma factor in rice and its tissue- and light-dependent expression. Nucleic Acids Res. 26:415-419 (1998) 
  
• Tanaka K, Tozawa Y, Mochizuki N, Shinozaki K, Nagatani A, Wakasa K, and Takahashi H. Characterization of three cDNA species encoding plastid RNA polymerase sigma factors in Arabidopsis thaliana: evidence for the sigma factor heterogeneity in higher plant plastids. FEBS Lett. 413:309-313 (1997) 
  
• Iwata M, Kuwata T, Mukai M, Tozawa Y, and Yokoyama Y. Differential induction of helper and killer T cells from isolated CD4+CD8+ thymocytes in suspension culture.  Eur. J. Immunol. 26:2081-2086 (1996) 
  
• Ohoka Y, Kuwata T, Tozawa Y, Zhao Y, Mukai M, Motegi Y, Suzuki R, Yokoyama M, and Iwata M. In vitro differentiation and commitment of CD4+ CD8+ thymocytes to the CD4 lineage, without TCR engagement. Int. Immunol. 8:297-306 (1996) 
  
• Sato TN, Tozawa Y, Deutsch U, Wolburg-Buchholz K, Fujiwara Y, Gendron-Maguire M, Gridley T, Wolburg H, Risau W, and Qin Y. Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. Nature 376: 70-74 (1995)
  
• Zhao Y, Tozawa Y, Iseki R, Mukai M, and Iwata M. Calcineurin activation protects T cells from glucocorticoid-induced apoptosis. J. Immunol. 154:6346-6354 (1995) 
  
• Iwata M, Iseki R, Sato K, Tozawa Y, and Ohoka Y. Involvement of protein kinase C-epsilon in glucocorticoid-induced apoptosis in thymocytes. Int. Immunol. 6:431-438 (1994) 
  
• Tozawa Y, Yoshikawa H, Kawamura F, Itaya M, and Takahashi H. Isolation and characterization of the groES and groEL genes of Bacillus subtilis Marburg. Biosci. Biotechnol. Biochem. 56:1995-2002 (1992) 
  
• Yamazaki S, Kobayashi N, Okubo A, Sato T, Tozawa Y, Yoshimura E, Kajiwara H, Hirano H, and Toda S. Charactreization of dimethylsulfoxide reductase, a novel molybdo-enzyme from photosynthetic bacteria, Rhodobacter spheroids f.s. denitrificans. Analytical Sciences 7:821-824 (1991)   

　総説・解説・プロシーディングス 

• Komatsu A, Yoshikawa A, Hasegawa H, Kawagishi-Kobayashi M, Nishizawa Y, Sugimoto K, Tozawa Y, Wakasa K. Analysis of rice transformants with the mutated anthranilate synthase gene driven by five different promoters fro the development of selection marker. Plant Cell Physiol. 43:S121 (2002)
• Tozawa Y, Tanaka K, Wakasa K. Molecular cloning of the gene encoding putative chloroplast RNA polymerase sigma subunit from rice. Plant Physiol. 114:1276 (1997)
• Crouch RJ, Tozawa Y, and Cerritelli SM. Ribonuclease H of Saccharomyces pombe and Crithithidia fasciculate share a common moth in their non-RNase-H domain that binds double-stranded-RNA in a magnesium-dependent manner. Protein Engineering 7:1151-1152 (1994)
• Tozawa Y, and Sato TN. Roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation. EXPERIMENTAL MEDICINE (実験医学) 14:88-91 (1996)

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